Vaspin对棕榈酸诱导的3T3-L1脂肪细胞胰岛素抵抗的影响及机制

Q4 Nursing

中华临床营养杂志 Pub Date : 2018-08-30 DOI:10.3760/CMA.J.ISSN.1674-635X.2018.04.006

Shiwei Liu, Ruixue Duan, Jiaxin Zhang, Yaru Wu, Xin Li, Shenghui Guo, Meimei Wang, Tao Yuan, Z. Shen

{"title":"Vaspin对棕榈酸诱导的3T3-L1脂肪细胞胰岛素抵抗的影响及机制","authors":"Shiwei Liu, Ruixue Duan, Jiaxin Zhang, Yaru Wu, Xin Li, Shenghui Guo, Meimei Wang, Tao Yuan, Z. Shen","doi":"10.3760/CMA.J.ISSN.1674-635X.2018.04.006","DOIUrl":null,"url":null,"abstract":"Objective \nTo investigate the effects of vaspin on insulin resistants of 3T3-L1 adipocyte through the insulin receptor substrates (IRS)/phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt)/glucose transporter (Glut) signaling pathway. \n \n \nMethods \n3T3-L1 cells cultured by palmitic acid (PA) were used to establish insulin resistance models, which were divided into PA group, PA+ 100 ng/ml vaspin group, PA+ 200 ng/ml vaspin group, PA+ 400 ng/ml vaspin group and PA+ 400 ng/ml vaspin+ wortmannin (PI3K inhibitor) group. Glucose uptake and consumption were assessed by 2-deoxy H3-D-glucose incorporation and glucose oxidase-peroxidase respectively. IRS/PI3K/Akt/Glut signaling pathway was evaluated using reverse transcription polymerase chain reaction and Western blot analysis. \n \n \nResults \nCompared with PA group, glucose uptake and consumption increased gradually with the increasing of vaspin concentration in other groups (P<0.05). mRNA levels of IRS-1, Akt and Glut 4 increased gradually as vaspin concentration increasing (P<0.05), and the ratios of p-IRS-1 to IRS-1, p-Akt to Akt and Glut 4 protein level also showed the same trends (P<0.05). However, glucose uptake and consumption in PA+ 400 ng/ml vaspin+ wortmannin group were less than that of PA+ 400 ng/ml vaspin group (P<0.05). PA+ 400 ng/ml vaspin+ wortmannin group showed lower mRNA and protein phosphorylation levels of IRS-1, Akt and Glut 4 (P<0.05), and that the ratios of p-IRS-1 to IRS-1, p-Akt to Akt and Glut 4 protein levels decreased (P<0.05). \n \n \nConclusions \nVaspin can improve the insulin sensitivity of 3T3-L1 adipocyte by activating IRS/PI3K/Akt/Glut signaling pathway. \n \n \nKey words: \nVaspin; Insulin resistance; 3T3-L1 adipocyte; IRS/PI3K/Akt/Glut","PeriodicalId":9877,"journal":{"name":"中华临床营养杂志","volume":"26 1","pages":"228-234"},"PeriodicalIF":0.0000,"publicationDate":"2018-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect and mechanism of Vaspin on insulin resistance of 3T3-L1 adipocytes induced by palmitic acid\",\"authors\":\"Shiwei Liu, Ruixue Duan, Jiaxin Zhang, Yaru Wu, Xin Li, Shenghui Guo, Meimei Wang, Tao Yuan, Z. Shen\",\"doi\":\"10.3760/CMA.J.ISSN.1674-635X.2018.04.006\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective \\nTo investigate the effects of vaspin on insulin resistants of 3T3-L1 adipocyte through the insulin receptor substrates (IRS)/phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt)/glucose transporter (Glut) signaling pathway. \\n \\n \\nMethods \\n3T3-L1 cells cultured by palmitic acid (PA) were used to establish insulin resistance models, which were divided into PA group, PA+ 100 ng/ml vaspin group, PA+ 200 ng/ml vaspin group, PA+ 400 ng/ml vaspin group and PA+ 400 ng/ml vaspin+ wortmannin (PI3K inhibitor) group. Glucose uptake and consumption were assessed by 2-deoxy H3-D-glucose incorporation and glucose oxidase-peroxidase respectively. IRS/PI3K/Akt/Glut signaling pathway was evaluated using reverse transcription polymerase chain reaction and Western blot analysis. \\n \\n \\nResults \\nCompared with PA group, glucose uptake and consumption increased gradually with the increasing of vaspin concentration in other groups (P<0.05). mRNA levels of IRS-1, Akt and Glut 4 increased gradually as vaspin concentration increasing (P<0.05), and the ratios of p-IRS-1 to IRS-1, p-Akt to Akt and Glut 4 protein level also showed the same trends (P<0.05). However, glucose uptake and consumption in PA+ 400 ng/ml vaspin+ wortmannin group were less than that of PA+ 400 ng/ml vaspin group (P<0.05). PA+ 400 ng/ml vaspin+ wortmannin group showed lower mRNA and protein phosphorylation levels of IRS-1, Akt and Glut 4 (P<0.05), and that the ratios of p-IRS-1 to IRS-1, p-Akt to Akt and Glut 4 protein levels decreased (P<0.05). \\n \\n \\nConclusions \\nVaspin can improve the insulin sensitivity of 3T3-L1 adipocyte by activating IRS/PI3K/Akt/Glut signaling pathway. \\n \\n \\nKey words: \\nVaspin; Insulin resistance; 3T3-L1 adipocyte; IRS/PI3K/Akt/Glut\",\"PeriodicalId\":9877,\"journal\":{\"name\":\"中华临床营养杂志\",\"volume\":\"26 1\",\"pages\":\"228-234\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-08-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中华临床营养杂志\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3760/CMA.J.ISSN.1674-635X.2018.04.006\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Nursing\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中华临床营养杂志","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3760/CMA.J.ISSN.1674-635X.2018.04.006","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Nursing","Score":null,"Total":0}

引用次数: 0

摘要

目的探讨vaspin通过胰岛素受体底物(IRS)/磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B (Akt)/葡萄糖转运蛋白(Glut)信号通路对3T3-L1脂肪细胞胰岛素抵抗的影响。方法采用棕榈酸(PA)培养的3T3-L1细胞建立胰岛素抵抗模型，分为PA组、PA+ 100 ng/ml vaspin组、PA+ 200 ng/ml vaspin组、PA+ 400 ng/ml vaspin组和PA+ 400 ng/ml vaspin+ wortmannin (PI3K抑制剂)组。葡萄糖摄取和消耗分别用2-脱氧h3 -d葡萄糖掺入和葡萄糖氧化酶-过氧化物酶测定。采用逆转录聚合酶链反应和Western blot分析IRS/PI3K/Akt/Glut信号通路。结果与PA组比较，其余各组大鼠葡萄糖摄取和消耗随血管素浓度的升高而逐渐升高(P<0.05)。随着vaspin浓度的升高，IRS-1、Akt和Glut - 4 mRNA水平逐渐升高(P<0.05)， P -IRS-1 / IRS-1、P -Akt / Akt和Glut - 4蛋白水平的比值也呈相同趋势(P<0.05)。而PA+ 400 ng/ml vaspin+ wortmannin组葡萄糖摄取和消耗均低于PA+ 400 ng/ml vaspin组(P<0.05)。PA+ 400 ng/ml vaspin+ wortmannin组小鼠IRS-1、Akt和Glut - 4 mRNA和蛋白磷酸化水平降低(P<0.05)， P -IRS-1 / IRS-1、P -Akt / Akt和Glut - 4蛋白水平降低(P<0.05)。结论Vaspin可通过激活IRS/PI3K/Akt/Glut信号通路改善3T3-L1脂肪细胞的胰岛素敏感性。关键词:Vaspin;胰岛素抵抗;3 t3-l1脂肪细胞;国税局/ PI3K / Akt /过剩

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Effect and mechanism of Vaspin on insulin resistance of 3T3-L1 adipocytes induced by palmitic acid

Objective To investigate the effects of vaspin on insulin resistants of 3T3-L1 adipocyte through the insulin receptor substrates (IRS)/phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt)/glucose transporter (Glut) signaling pathway. Methods 3T3-L1 cells cultured by palmitic acid (PA) were used to establish insulin resistance models, which were divided into PA group, PA+ 100 ng/ml vaspin group, PA+ 200 ng/ml vaspin group, PA+ 400 ng/ml vaspin group and PA+ 400 ng/ml vaspin+ wortmannin (PI3K inhibitor) group. Glucose uptake and consumption were assessed by 2-deoxy H3-D-glucose incorporation and glucose oxidase-peroxidase respectively. IRS/PI3K/Akt/Glut signaling pathway was evaluated using reverse transcription polymerase chain reaction and Western blot analysis. Results Compared with PA group, glucose uptake and consumption increased gradually with the increasing of vaspin concentration in other groups (P<0.05). mRNA levels of IRS-1, Akt and Glut 4 increased gradually as vaspin concentration increasing (P<0.05), and the ratios of p-IRS-1 to IRS-1, p-Akt to Akt and Glut 4 protein level also showed the same trends (P<0.05). However, glucose uptake and consumption in PA+ 400 ng/ml vaspin+ wortmannin group were less than that of PA+ 400 ng/ml vaspin group (P<0.05). PA+ 400 ng/ml vaspin+ wortmannin group showed lower mRNA and protein phosphorylation levels of IRS-1, Akt and Glut 4 (P<0.05), and that the ratios of p-IRS-1 to IRS-1, p-Akt to Akt and Glut 4 protein levels decreased (P<0.05). Conclusions Vaspin can improve the insulin sensitivity of 3T3-L1 adipocyte by activating IRS/PI3K/Akt/Glut signaling pathway. Key words: Vaspin; Insulin resistance; 3T3-L1 adipocyte; IRS/PI3K/Akt/Glut

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

中华临床营养杂志 Nursing-Nutrition and Dietetics

CiteScore

0.20

自引率

0.00%

发文量

2282

期刊介绍： The Chinese Journal of Clinical Nutrition was founded in 1993. It is the first professional academic journal (bimonthly) in my country co-sponsored by the Chinese Medical Association and the Chinese Academy of Medical Sciences to disseminate information on clinical nutrition support, nutrient metabolism, the impact of nutrition support on outcomes and "cost-effectiveness", as well as translational medicine and nutrition research. It is also a professional journal of the Chinese Medical Association's Parenteral and Enteral Nutrition Branch. The purpose of the Chinese Journal of Clinical Nutrition is to promote the rapid dissemination of knowledge on nutrient metabolism and the rational application of parenteral and enteral nutrition, focusing on the combination of multidisciplinary and multi-regional field investigations and clinical research. It mainly reports on nutritional risk screening related to the indications of parenteral and enteral nutrition support, "cost-effectiveness" research on nutritional drugs, consensus on clinical nutrition, guidelines, expert reviews, randomized controlled studies, cohort studies, glycoprotein and other nutrient metabolism research, systematic evaluation of clinical research, evidence-based case reports, special reviews, case reports and clinical experience exchanges, etc., and has a special column on new technologies related to the field of clinical nutrition and their clinical applications.