两种保存方法保存的钩端螺旋体参考菌株遗传稳定性评价

NOVA Pub Date : 2022-12-21 DOI:10.22490/24629448.6585
Oscar Gabriel Beltrán, Ligia D Torres Higuera, J. L. Rodríguez Bautista, Rocio E Patiño Burbano
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引用次数: 0

摘要

客观的对两个保护系统下钩端螺旋体菌株的遗传稳定性进行了评价。方法论测定了钩端螺旋体属(SBGNAA)的10个参考血清型的16S rRNA和ompL1基因的保守性。后果证实了评估的基因没有发生显著的变化,因为在配对比对中,16S rRNA和ompL1的相似性分别大于99.69%和99.02%。结论验证了钩端螺旋体参考菌株的遗传稳定性和纯度。spp.,在-196°C的液氮和室温下冷冻保存约8年。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of the genetic stability of Leptospira reference strains maintained under two conservation methods
Objective. The genetic stability of Strains of Leptospira spp., maintained under two conservation systems, was evaluated. Methodology. The degree of conservation of the 16S rRNA and ompL1 genes of 10 reference serovars from the Leptospira spp. collection, belonging to the Sistema de Bancos de Germoplasma de la Nación para la Alimentación y la Agricultura (SBGNAA), was determined. Results. It was corroborated that the genes evaluated these have not undergone considerable changes, since similarities greater than 99.69 % were evidenced for 16S rRNA and 99.02% for ompL1, in the paired alignments. Conclusion. The genetic stability and purity of the reference strains of Leptospira spp. were verified. spp., kept in cryopreservation in liquid nitrogen at -196 °C and at room temperature for approximately eight years.
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