{"title":"黄藻和红藻从鳐岛的水域对其进行的抗氧化剂的特性","authors":"Elvi Kustiyah, Bungaran Saing, Avira Afriyanti, Ibnu Susanto Joyosemito","doi":"10.26760/jrh.v2i3.2513","DOIUrl":null,"url":null,"abstract":"ABSTRAKKandungan dalam alga meliputi polisakarida, mineral, protein, lemak, vitamin, polifenol dan senyawa bioaktif yang bisa memberikan manfaat terhadap sumber makanan, vitamin maupun pharmasi. Investigasi karakter Alga Coklat dan Alga Merah yang diperoleh dari perairan pulau pari kepulauan seribu wilayah Indonesia dilakukan dengan tujuan untuk mendapatkan zat yang mampu menjadi alternatif sumber anti oksidan . Metode yang digunakan dalam penelitian ini adalah ekstraksi maserasi dengan pelarut Phosfat Buffer Saline (PBS) sedangkan Uji kadar protein alga menggunakan metode Lowry dan uji aktivitas antioksidan menggunakan metode 1,1-Diphenyl-2-Pycrylhydrazy(DPPH). Hasil penelitian menunjukkan kadar protein yang tertinggi diperoleh pada sampel alga merah, sedangkan nilai kadar protein paling rendah terdapat pada alga cokelat . Nilai aktivitas antioksidan yang paling baik yaitu pada sampel yang berasal dari alga merah dengan nilai IC50 sebesar 3,5421 mg/ml.Kata Kunci: Alga, protein, antioksidan.ABSTRACTAn alga contains polysaccharides, minerals, proteins, fats, vitamins, polyphenols and bioactive compounds that can provide benefits for food, vitamin and pharmaceutical sources. Characteristic investigations of Brown Algae and Red Algae from the Pari Island waters, Thousand Islands, Indonesia, were carried out to obtain substances that could be an alternative source of antioxidants. The maceration extraction method using Phosphate Buffered Saline (PBS) as solvent was used in this study. Protein content in algae was investigated by Lowry method and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method was used to investigate the antioxidant activity of algae The results showed that the highest protein content was found in Red Algae and the lowest is in Brown Algae. The best value of antioxidant activity was found in Red Algae with the IC50 value of 3.5421 mg/ml.Keywords: Algae, protein, antioxidants.","PeriodicalId":34848,"journal":{"name":"Rekayasa Hijau Jurnal Teknologi Ramah Lingkungan","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2018-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Karakterisasi Protein Alga Coklat dan Merah dari Perairan Pulau Pari Sebagai Zat Antioksidan\",\"authors\":\"Elvi Kustiyah, Bungaran Saing, Avira Afriyanti, Ibnu Susanto Joyosemito\",\"doi\":\"10.26760/jrh.v2i3.2513\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"ABSTRAKKandungan dalam alga meliputi polisakarida, mineral, protein, lemak, vitamin, polifenol dan senyawa bioaktif yang bisa memberikan manfaat terhadap sumber makanan, vitamin maupun pharmasi. Investigasi karakter Alga Coklat dan Alga Merah yang diperoleh dari perairan pulau pari kepulauan seribu wilayah Indonesia dilakukan dengan tujuan untuk mendapatkan zat yang mampu menjadi alternatif sumber anti oksidan . Metode yang digunakan dalam penelitian ini adalah ekstraksi maserasi dengan pelarut Phosfat Buffer Saline (PBS) sedangkan Uji kadar protein alga menggunakan metode Lowry dan uji aktivitas antioksidan menggunakan metode 1,1-Diphenyl-2-Pycrylhydrazy(DPPH). Hasil penelitian menunjukkan kadar protein yang tertinggi diperoleh pada sampel alga merah, sedangkan nilai kadar protein paling rendah terdapat pada alga cokelat . Nilai aktivitas antioksidan yang paling baik yaitu pada sampel yang berasal dari alga merah dengan nilai IC50 sebesar 3,5421 mg/ml.Kata Kunci: Alga, protein, antioksidan.ABSTRACTAn alga contains polysaccharides, minerals, proteins, fats, vitamins, polyphenols and bioactive compounds that can provide benefits for food, vitamin and pharmaceutical sources. Characteristic investigations of Brown Algae and Red Algae from the Pari Island waters, Thousand Islands, Indonesia, were carried out to obtain substances that could be an alternative source of antioxidants. The maceration extraction method using Phosphate Buffered Saline (PBS) as solvent was used in this study. Protein content in algae was investigated by Lowry method and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method was used to investigate the antioxidant activity of algae The results showed that the highest protein content was found in Red Algae and the lowest is in Brown Algae. The best value of antioxidant activity was found in Red Algae with the IC50 value of 3.5421 mg/ml.Keywords: Algae, protein, antioxidants.\",\"PeriodicalId\":34848,\"journal\":{\"name\":\"Rekayasa Hijau Jurnal Teknologi Ramah Lingkungan\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-12-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Rekayasa Hijau Jurnal Teknologi Ramah Lingkungan\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.26760/jrh.v2i3.2513\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Rekayasa Hijau Jurnal Teknologi Ramah Lingkungan","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.26760/jrh.v2i3.2513","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Karakterisasi Protein Alga Coklat dan Merah dari Perairan Pulau Pari Sebagai Zat Antioksidan
ABSTRAKKandungan dalam alga meliputi polisakarida, mineral, protein, lemak, vitamin, polifenol dan senyawa bioaktif yang bisa memberikan manfaat terhadap sumber makanan, vitamin maupun pharmasi. Investigasi karakter Alga Coklat dan Alga Merah yang diperoleh dari perairan pulau pari kepulauan seribu wilayah Indonesia dilakukan dengan tujuan untuk mendapatkan zat yang mampu menjadi alternatif sumber anti oksidan . Metode yang digunakan dalam penelitian ini adalah ekstraksi maserasi dengan pelarut Phosfat Buffer Saline (PBS) sedangkan Uji kadar protein alga menggunakan metode Lowry dan uji aktivitas antioksidan menggunakan metode 1,1-Diphenyl-2-Pycrylhydrazy(DPPH). Hasil penelitian menunjukkan kadar protein yang tertinggi diperoleh pada sampel alga merah, sedangkan nilai kadar protein paling rendah terdapat pada alga cokelat . Nilai aktivitas antioksidan yang paling baik yaitu pada sampel yang berasal dari alga merah dengan nilai IC50 sebesar 3,5421 mg/ml.Kata Kunci: Alga, protein, antioksidan.ABSTRACTAn alga contains polysaccharides, minerals, proteins, fats, vitamins, polyphenols and bioactive compounds that can provide benefits for food, vitamin and pharmaceutical sources. Characteristic investigations of Brown Algae and Red Algae from the Pari Island waters, Thousand Islands, Indonesia, were carried out to obtain substances that could be an alternative source of antioxidants. The maceration extraction method using Phosphate Buffered Saline (PBS) as solvent was used in this study. Protein content in algae was investigated by Lowry method and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method was used to investigate the antioxidant activity of algae The results showed that the highest protein content was found in Red Algae and the lowest is in Brown Algae. The best value of antioxidant activity was found in Red Algae with the IC50 value of 3.5421 mg/ml.Keywords: Algae, protein, antioxidants.