乳及部分乳制甜点肠球菌多药耐药及毒力因子研究

Q3 Agricultural and Biological Sciences

Journal of Food Quality and Hazards Control Pub Date : 2022-12-14 DOI:10.18502/jfqhc.9.4.11376

O. Sadek, A. Koriem

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引用次数: 2

摘要

背景:肠球菌属细菌，特别是粪肠球菌和粪肠球菌具有获得抗生素耐药模式和引起危及生命的医院获得性感染的能力。因此，本研究的目的是计数和分离埃及Assiut市消费的牛奶和乳制品甜点中的粪肠球菌和粪肠球菌。方法:采集埃及Assiut市奶牛场100份原料奶、冰淇淋、美hallabia和乳米样品，对肠球菌进行细菌学检测和计数，并采用常规方法和聚合酶链式反应(PCR)法对肠球菌分离株进行鉴定，进行抗生素敏感性试验，对多重耐药(MDR)分离株进行毒力基因鉴定。结果:原料奶、冰淇淋、麦大拉菌和乳米样品中肠球菌计数检出率分别为76、44、20和32%。原料奶、冰淇淋、沼虾和乳米样品中粪肠杆菌的检出率分别为64%、0%、0%和8%，粪肠杆菌的检出率分别为12%、44%、20%和24%。对万古霉素、环丙沙星、庆大霉素、红霉素和四环素的耐药率分别为72.2%、88.9、88.9、94.4%和77.8%，对粪肠杆菌的耐药率分别为16%、40%、16%、84%和20%。粪肠杆菌和粪肠杆菌的耐药率分别为88.9和32%。结论:本研究表明，牛奶、冰淇淋、米哈拉比亚菌和乳米可能是埃及Assiut消费者肠球菌的来源。粪肠杆菌的耐药指数和耐药指数均高于粪肠杆菌。

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Multidrug Resistance and Virulence Factors of Enterococci Isolated from Milk and Some Dairy Desserts

Background: Enterococci spp. bacteria especially Enterococcus faecalis and E. faecium have the ability to acquire antibiotic-resistance pattern and causing life-threatening hospital-acquired infections. So, the aim of this study was to count and isolate of E. faecalis and E. faecium from milk and dairy desserts consumed in Assiut city, Egypt. Methods: A total of 100 raw milk, ice cream, mehallabia, and milk rice samples were collected from dairies shop in Assiut city, Egypt and were bacteriologically examined for the presence and count of Enterococcus spp. Then, identification of enterococci isolates by conventional and Polymerase Chain Reaction (PCR) methods, performance of antibiotic sensitivity assay, and some virulence genes in the Multi Drug Resistant (MDR) isolates were identified. Results: The prevalence of counted Enterococcus spp. in raw milk, ice cream, mehallabia, and milk rice samples were 76, 44, 20, and 32%, respectively. The prevalence of E. faecalis in raw milk, ice cream, mehallabia, and milk rice samples were 64, 0, 0, and 8%, while for E. faecium were 12, 44, 20, and 24%, respectively. E. faecalis isolates were resistant to vancomycin, ciprofloxacin, gentamicin, erythromycin, and tetracycline with the rate of 72.2, 88.9, 88.9, 94.4, and 77.8%, respectively, while for the resistance rates of E. faecium were 16, 40, 16, 84, and 20%, respectively. E. faecalis and E. faecium were MDR in rate of 88.9 and 32%, respectively. Conclusion: This study revealed that milk, ice cream, mehallabia, and milk rice could be a source of enterococci to consumers in Assiut, Egypt. Moreover, E. faecalis had higher MDR and Resistant Index (RI) than E. faecium.

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来源期刊

Journal of Food Quality and Hazards Control Agricultural and Biological Sciences-Food Science

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

8 weeks

期刊介绍： Journal of Food Quality and Hazards Control (J. Food Qual. Hazards Control) is an international peer-reviewed quarterly journal that aims at publishing of high quality articles involved in food quality, food hygiene, food safety, and food control which scientists from all over the world may submit their manuscript. This academic journal aims to improve international exchange of new findings and recent developments in all aspects of agricultural and biological sciences. This free of charge journal is published in both online and print forms and welcomes the manuscripts that fulfill the general criteria of novelty and scientific importance. Among the most significant objectives of Journal of Food Quality and Hazards Control are to ensure that the articles reflect a wide range of topics regarding journal scopes; to do a fair, scientific, fast, as well as high quality peer-review process; to provide a wide and diverse geographical coverage of articles around the world; and to publish the articles having a trustable resource of scientific information for the audiences. The types of acceptable submissions include original article, review article, short communication, letter to the editor, case report, editorial, as well as book review. Journal of Food Quality and Hazards Control is an official journal of Research Center for Food Hygiene and Safety, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.