Davide Gerna, David Clara, Livio Antonielli, Birgit Mitter, Thomas Roach
{"title":"种子的吸胀和代谢对大豆全息体的初始组装有不同的贡献","authors":"Davide Gerna, David Clara, Livio Antonielli, Birgit Mitter, Thomas Roach","doi":"10.1094/PBIOMES-03-23-0019-MF","DOIUrl":null,"url":null,"abstract":"<p><p>Seed germination critically determines successful plant establishment and agricultural productivity. In the plant holobiont's life cycle, seeds are hubs for microbial communities' assembly, but what exactly shapes the holobiont during germination remains unknown. Here, 16S rRNA gene amplicon sequencing characterized the bacterial communities in embryonic compartments (cotyledons and axes) and on seed coats pre- and post-germination of four soybean (<i>Glycine max</i>) cultivars, in the presence or absence of exogenous abscisic acid (ABA), which prevented germination and associated metabolism of seeds that had imbibed. Embryonic compartments were metabolically profiled during germination to design minimal media mimicking the seed endosphere for bacterial growth assays. The distinction between embryonic and seed coat bacterial microbiomes of dry seeds weakened during germination, resulting in the plumule, radicle, cotyledon, and seed coat all hosting the same most abundant and structurally influential genera in germinated seeds of every cultivar. Treatment with ABA prevented the increase of bacterial microbiomes' richness, but not taxonomic homogenization across seed compartments. Growth assays on minimal media containing the most abundant metabolites that accumulated in germinated seeds revealed that seed reserve mobilization promoted enrichment of copiotrophic bacteria. Our data show that seed imbibition enabled distribution of seed-coat-derived epiphytes into embryos irrespective of germination, while germinative metabolism promoted proliferation of copiotrophic taxa, which predominated in germinated seeds.</p>","PeriodicalId":48504,"journal":{"name":"Phytobiomes Journal","volume":null,"pages":null},"PeriodicalIF":3.3000,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7616048/pdf/","citationCount":"0","resultStr":"{\"title\":\"Seed Imbibition and Metabolism Contribute Differentially to Initial Assembly of the Soybean Holobiont.\",\"authors\":\"Davide Gerna, David Clara, Livio Antonielli, Birgit Mitter, Thomas Roach\",\"doi\":\"10.1094/PBIOMES-03-23-0019-MF\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Seed germination critically determines successful plant establishment and agricultural productivity. In the plant holobiont's life cycle, seeds are hubs for microbial communities' assembly, but what exactly shapes the holobiont during germination remains unknown. Here, 16S rRNA gene amplicon sequencing characterized the bacterial communities in embryonic compartments (cotyledons and axes) and on seed coats pre- and post-germination of four soybean (<i>Glycine max</i>) cultivars, in the presence or absence of exogenous abscisic acid (ABA), which prevented germination and associated metabolism of seeds that had imbibed. Embryonic compartments were metabolically profiled during germination to design minimal media mimicking the seed endosphere for bacterial growth assays. The distinction between embryonic and seed coat bacterial microbiomes of dry seeds weakened during germination, resulting in the plumule, radicle, cotyledon, and seed coat all hosting the same most abundant and structurally influential genera in germinated seeds of every cultivar. Treatment with ABA prevented the increase of bacterial microbiomes' richness, but not taxonomic homogenization across seed compartments. Growth assays on minimal media containing the most abundant metabolites that accumulated in germinated seeds revealed that seed reserve mobilization promoted enrichment of copiotrophic bacteria. Our data show that seed imbibition enabled distribution of seed-coat-derived epiphytes into embryos irrespective of germination, while germinative metabolism promoted proliferation of copiotrophic taxa, which predominated in germinated seeds.</p>\",\"PeriodicalId\":48504,\"journal\":{\"name\":\"Phytobiomes Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2022-08-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7616048/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Phytobiomes Journal\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1094/PBIOMES-03-23-0019-MF\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2022/7/18 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Phytobiomes Journal","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1094/PBIOMES-03-23-0019-MF","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/7/18 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
种子发芽至关重要地决定着植物的成功建立和农业生产力。在植物全生物的生命周期中,种子是微生物群落组装的中心,但在发芽过程中,全生物的确切形状仍然未知。在这里,16S rRNA基因扩增子测序表征了在存在或不存在外源脱落酸(ABA)的情况下,四个大豆(Glycine max L.)品种发芽前后胚胎隔室(子叶和轴)和种皮上的细菌群落,ABA阻止了吸胀种子的发芽和相关代谢。在发芽过程中对胚胎区室进行代谢分析,以设计用于细菌生长测定的模拟种子内球体的最小培养基。干种子的胚胎和种皮细菌微生物组之间的区别在发芽过程中减弱,导致胚芽、胚根、子叶和种皮在每个品种的发芽种子中都拥有相同的最丰富和结构上最具影响力的属。ABA处理阻止了细菌微生物组丰富度的增加,但没有阻止种子区室的分类均匀化。在含有发芽种子中积累的最丰富代谢物的最小培养基上进行的生长试验表明,种子储备动员促进了共营养细菌的富集。我们的数据表明,无论发芽如何,种子吸收都能使种皮衍生的附生植物分布到胚胎中,而发芽代谢促进了共生类群的增殖,共生类群在发芽种子中占主导地位。
Seed Imbibition and Metabolism Contribute Differentially to Initial Assembly of the Soybean Holobiont.
Seed germination critically determines successful plant establishment and agricultural productivity. In the plant holobiont's life cycle, seeds are hubs for microbial communities' assembly, but what exactly shapes the holobiont during germination remains unknown. Here, 16S rRNA gene amplicon sequencing characterized the bacterial communities in embryonic compartments (cotyledons and axes) and on seed coats pre- and post-germination of four soybean (Glycine max) cultivars, in the presence or absence of exogenous abscisic acid (ABA), which prevented germination and associated metabolism of seeds that had imbibed. Embryonic compartments were metabolically profiled during germination to design minimal media mimicking the seed endosphere for bacterial growth assays. The distinction between embryonic and seed coat bacterial microbiomes of dry seeds weakened during germination, resulting in the plumule, radicle, cotyledon, and seed coat all hosting the same most abundant and structurally influential genera in germinated seeds of every cultivar. Treatment with ABA prevented the increase of bacterial microbiomes' richness, but not taxonomic homogenization across seed compartments. Growth assays on minimal media containing the most abundant metabolites that accumulated in germinated seeds revealed that seed reserve mobilization promoted enrichment of copiotrophic bacteria. Our data show that seed imbibition enabled distribution of seed-coat-derived epiphytes into embryos irrespective of germination, while germinative metabolism promoted proliferation of copiotrophic taxa, which predominated in germinated seeds.