庆大霉素致肾损伤后的肾再生及维生素C的降毒作用

Iranian Journal of Ichthyology Pub Date : 2019-03-25 DOI:10.22034/IJI.V6I1.351

M. Motamedi, A. Iranmanesh, A. Teimori

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引用次数: 3

摘要

已知庆大霉素可以刺激活性氧的产生，并通过氧化应激导致某些脊椎动物的肾脏损伤。研究了庆大霉素诱导的氧化应激对大鲵肾再生的影响。此外，我们评估了维生素C作为抗氧化剂在减少肾毒性中的可能作用。24个标本(总长度3.5 ~ 4cm)分为4组:1组2个鱼标本，注射生理盐水7.5µl;ii组10只，给予10µg/g体重(b.w)庆大霉素;在第三组，2只鱼被给予60µg/g b.w的维生素C;IV组，10个标本同时接受10µg/g庆大霉素和60µg/g b.w维生素c。在肾毒性阶段(即注射后10小时(hpi)至注射后5天(dpi))， i、III和IV组的肾脏组织每天采样10 dpi，直到再生完成。在第8 dpi时，新形成的肾元检测到树蛙通过肾元新生进行肾脏再生，在第10 dpi时完成肾元再生。庆大霉素诱导的氧化应激(ii组)的组织学结果清楚地表明，肾脏组织在1-4 dpi内发生最严重的损伤。然而，在提供维生素C (iv族)抗氧化治疗的标本中，庆大霉素的损伤作用在这些天内明显受到抑制。本研究的结果显示，维生素C的管理是有效的减少庆大霉素引起的肾毒性在A. furcatus。维生素C通过其抗氧化活性减少自由基和活性氧(ROS)，从而降低庆大霉素的毒性作用。因此，维生素C与庆大霉素联合使用可以增强机体对应激条件的抵抗力，降低庆大霉素的毒性作用。

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Kidney regeneration in Aphanius furcatus (Teleostei: Aphaniidae) after damage induced by gentamicin and evaluation of vitamin C in toxicity reduction

Gentamicin is known to stimulate the production of reactive oxygen species and lead to renal damage in some groups of vertebrates through oxidative stress. The kidney regeneration of the killifish Aphanius furcatus was studied after gentamicin-induced oxidative stress. In addition, we evaluated the possible role of vitamin C as an antioxidant in the reduction of renal toxicity. A total of 24 specimens (3.5-4cm in total length) were divided into four groups: In group-I two fish specimens treated with 7.5µl of normal saline injection; in group-II, 10 specimens received 10µg/g body weight (b.w) gentamicin; in group-III, two fish were treated with totally 60µg/g b.w vitamin C; and in group-IV, 10 specimens received both 10µg/g gentamicin and 60µg/g b.w vitamin C. The kidney tissues of group-I, III and IV were sampled during the nephrotoxicity phase (i.e., 10 hours post injection (hpi) to 5 days post injection (dpi)) and kidney tissues of group-II sampled daily for 10 dpi until regeneration completed. The kidney regeneration of A. furcatus through nephron neogenesis was detected by newly formed nephron on 8 dpi, and it is completed on 10 dpi. Our histological results in gentamicin-induced oxidative stress (group-II) clearly showed that the most severe damage is occurred in the kidney tissue within 1-4 dpi. However, in the specimens that antioxidant therapy supplied with vitamin C (group-IV), damage effect of gentamicin has remarkably repressed within these days. The outcome of this study revealed that administration of vitamin C is effective in reduction of gentamicin-induced nephrotoxicity in A. furcatus . Vitamin C reduces the toxicity effect of gentamicin by reduction of free radicals and reactive oxygen species (ROS) through its antioxidant activity. Therefore, using vitamin C together with gentamicin enhances the body resistance to the stress condition and reduces the toxicity effect of gentamicin.

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来源期刊

Iranian Journal of Ichthyology Agricultural and Biological Sciences-Aquatic Science

CiteScore

0.70

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0.00%

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期刊介绍： Iranian Journal of Ichthyology (IJI) is a peer-reviewed journal for publication of high quality papers on any aspect of ichthyology and will be published 4 times a year by the Iranian Society of Ichthyology (http://www.isi-org.ir). The journal welcomes the submission of manuscripts that meets the general criteria of significance and scientific excellence, and will publish original scientific articles in the fields of ichthyology. The editors welcome the submission of original manuscripts including Research Articles, Short communications, and Views & News. Review papers by distinguished authors and Special Issues will also be considered. It is our aim to provide the international scientific community with an efficiently published journal, meeting high scientific and technical standards. Scopes of Iranian Journal of Ichthyology includes: Systematics, taxonomy, morphology, anatomy, biogeography, biodiversity, conservation, ecology and general biology of fishes (reproduction, food and feeding habits, age and growth, population dynamics, etc.). Papers on freshwater, brackish, marine and fossil fishes will be considered.