生物膜清洗剂与多酶清洗剂对内镜生物膜清洗效果的比较

中华消化内镜杂志 Pub Date : 2020-01-20 DOI:10.3760/CMA.J.ISSN.1007-5232.2020.01.008

Xia Guili, W. Cai, Wei Gong, Fachao Zhi, Xi Huang, Xiaxi Li, Lei Zhengxia, D. Ling

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引用次数: 0

摘要

目的探讨生物膜清洗剂和两种多酶清洗剂对内镜下生物膜的清洗效果。方法采用铜绿假单胞菌建立内镜下生物膜模型，分别用1号多酶洗涤剂、2号多酶洗涤剂和生物膜清洗剂浸泡。对照组用无菌水清洗。在室温下放置5、10、15分钟后，采用细菌计数法和扫描电镜对清洗效果进行评价。采用Arova法比较各组间活菌数。结果浸泡5、10、15 min时，1号多酶洗涤剂组生物膜标准菌落计数(CFU/cm2)分别为5.31±0.10、5.04±0.08、4.90±0.16,2号多酶洗涤剂组为5.53±0.30、5.39±0.21、5.03±0.42，生物膜清洗剂组为3.53±0.30、3.01±0.07、2.82±0.26，空白对照组为7.92±0.21。两组间菌落数量无显著差异(P < 0.05)。但生物膜清洗剂组菌落数量少于两组多酶洗涤剂组(P<0.05)，且随时间延长菌落数量减少(P<0.05)。扫描电镜下，生物膜清洗剂组生物膜和细菌残留量最小。结论生物膜清洗剂能显著提高内镜清洗质量，值得临床推广。关键词:内窥镜;生物膜;生物膜清洗剂;工艺等洗涤剂

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Comparison of biofilm cleaning agent and multi-enzyme detergent on endoscopic biofilm cleaning effects

Objective To investigate the cleaning effects of biofilm cleaning agent and two kinds of multi-enzyme detergents on endoscopic biofilm. Methods Endoscopic biofilm model was established using pseudomonas aeruginosa, and soaked with No. 1 multi-enzyme detergent, No. 2 multi-enzyme detergent, and biofilm cleaning agent respectively. The control group was cleaned with sterile water. After 5, 10, and 15 minutes at room temperature, the cleaning effects were evaluated by bacteria counting method and scanning electron microscope. Arova was used for the comparison of viable counts among groups. Results At 5, 10, and 15 minutes of soak, the standard colony counts (CFU/cm2) of biofilm was 5.31±0.10, 5.04±0.08 and 4.90±0.16 in the No.1 multi-enzyme detergent group, 5.53±0.30, 5.39±0.21 and 5.03±0.42 in the No.2 multi-enzyme detergent group, and 3.53±0.30, 3.01±0.07 and 2.82±0.26 in the biofilm cleaning agent group, and 7.92±0.21 in the blank control group. There was no significant difference in the colony counts between the two multi-enzyme detergent groups (P>0.05). However, the colony counts of biofilm cleaning agent group was less than that of the two multi-enzyme detergent groups (P<0.05), and decreased with time (P<0.05). Under scanning electron microscope, the biofilm cleaning agent group had the least residual biofilm and bacteria. Conclusion Biofilm cleaning agent can significantly improve the quality of endoscopic cleaning, and is worthy of clinical promotion. Key words: Endoscopes; Biofilms; Biofilm cleaning agent; Multi-enzyme detergent

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来源期刊

中华消化内镜杂志

CiteScore

0.10

自引率

0.00%

发文量

7555

期刊介绍： Chinese Journal of Digestive Endoscopy is a high-level medical academic journal specializing in digestive endoscopy, which was renamed Chinese Journal of Digestive Endoscopy in August 1996 from Endoscopy. Chinese Journal of Digestive Endoscopy mainly reports the leading scientific research results of esophagoscopy, gastroscopy, duodenoscopy, choledochoscopy, laparoscopy, colorectoscopy, small enteroscopy, sigmoidoscopy, etc. and the progress of their equipments and technologies at home and abroad, as well as the clinical diagnosis and treatment experience. The main columns are: treatises, abstracts of treatises, clinical reports, technical exchanges, special case reports and endoscopic complications. The target readers are digestive system diseases and digestive endoscopy workers who are engaged in medical treatment, teaching and scientific research. Chinese Journal of Digestive Endoscopy has been indexed by ISTIC, PKU, CSAD, WPRIM.