CRISPR系统：有什么新的，下一步在哪里？

Q4 Biochemistry, Genetics and Molecular Biology

Biochemist Pub Date : 2021-12-15 DOI:10.1042/bio_2021_194

Ashley Parkes, Fiona E Kemm, Liu He, Tom Killelea

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引用次数: 0

摘要

天然CRISPR-Cas系统的遗传特征首次出现在1989年的一份出版物中，并在2002年至2007年进行了详细表征，最终首次报道了原核适应性免疫系统。从那时起，CRISPR-Cas酶已被应用于分子生物学工具中，这些工具改变了生命各个领域的基因工程。在这篇专题文章中，我们描述了CRISPR-Cas酶在基因工程中的起源、用途和未来：我们强调了过去10年取得的进展。这些进展的核心是对CRISPR工程和DNA修复之间相互作用的认识。我们强调了这种关系是如何被操纵的，以在基因编辑的发展中创造进一步的进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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CRISPR systems: what’s new, where next?

The genetic signature of natural CRISPR-Cas systems were first noted in a 1989 publication and were characterized in detail from 2002 to 2007, culminating in the first report of a prokaryotic adaptive immune system. Since then, CRISPR-Cas enzymes have been adapted into molecular biology tools that have transformed genetic engineering across domains of life. In this feature article, we describe origins, uses and futures of CRISPR-Cas enzymes in genetic engineering: we highlight advances made in the past 10 years. Central to these advances is appreciation of interplay between CRISPR engineering and DNA repair. We highlight how this relationship has been manipulated to create further advances in the development of gene editing.

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来源期刊

Biochemist Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

1.20

自引率

0.00%

发文量

期刊介绍： This lively and eclectic magazine for all life scientists appears six times a year. Its quirky style and astute selection of serious and humorous articles ensures that the magazine"s appeal is by no means restricted to that of the avid biochemist. Specially commissioned articles from leading scientists bring a popular science perspective direct to you! Forthcoming themes include: RNAi, Money in Science, Extremophiles, Biosystems and Mathematical Modelling, Renascence of Mitochondria, Prions & Protein factors, Imaging live cells and Model organisms.