人参皂苷Rg1对小鼠骨髓间充质干细胞MDA抑制增殖的保护作用及其机制

Ye Li, Cheng Ma, Z. Lv, Changhai Shao, Jun Zhang, Wenye Geng, Lan Zheng
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引用次数: 0

摘要

目的:研究人参皂苷Rg1 (GS-Rg1)对丙二醛(MDA)抑制间充质干细胞(MSCs)增殖的体外保护作用及其可能机制。方法:用GS-Rg1(10、50、100[公式:见文]mg/L)处理小鼠骨髓源性间充质干细胞24 h后,与MDA体外孵育,检测CFU-Fassay、细胞活力和BrdU结合实验,采用Q-RT-PCR和Western blotting检测MSC细胞周期蛋白依赖性激酶2 (CDK2)、p21和细胞周期蛋白E的表达。结果:GS-Rg1预处理小鼠骨髓间充质干细胞后,小鼠骨髓间充质干细胞的集落数量、大小、集落形成细胞数量、甲基噻唑四氮唑(MTT)吸收值和brdu阳性细胞比例均显著增加。GS-Rg1预处理可显著降低p21的表达水平,增加CDK2和cyclin e的表达,使MSCs免受mda抑制的增殖。结论:其保护机制可能与其提高CDK2和cyclin E的表达,降低p21的表达有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Protective effect and mechanisms of ginsenoside Rg1 against MDA-suppressed proliferation in mesenchymal stem cells derived from murine bone marrow
Objective: The present study was designed to investigate the cytoprotective effects of ginsenoside Rg1 (GS-Rg1) against malondialdehyde (MDA)-suppressed proliferation of the mesenchymal stem cells (MSCs) and its possible mechanisms in vitro. Methods: Murine bone marrow-derived MSCs were treated with GS-Rg1 (10, 50, 100[Formula: see text]mg/L) for 24[Formula: see text]h before being incubated with MDA in vitro, CFU-Fassay, the cell viability and BrdU incorporation assay were examined, the expression of cyclin-dependent kinase 2 (CDK2), p21 and cyclin E of MSC were examined by Q-RT-PCR and Western blotting. Results: The results showed that the number and size of murine bone marrow MSC colonies, the number of colony-forming cells, methyl thiazolyltetrazolium (MTT) absorbed value greatly and percentage of BrdU-positive cells increased significantly in MSC pretreated with GS-Rg1. GS-Rgl pretreatment markedly decreased the expression level of p21 and increased the expression of CDK2 and cyclin E. GS-Rg1 protects MSCs from MDA-suppressed proliferation. Conclusion: The protective mechanism could be related to its ability to increase the expression of CDK2 and cyclin E, and to reduce the expression of p21.
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