脲酶产生菌的分离鉴定及其沉淀碳酸钙的能力

Q3 Pharmacology, Toxicology and Pharmaceutics

Defence Life Science Journal Pub Date : 2022-06-17 DOI:10.14429/dlsj.7.17279

D. B. Khambholja, Dharmishtha Gamit, H. Patel, Prashant Kumar

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引用次数: 0

摘要

本研究旨在分离和表征产脲酶的细菌，并评估其沉淀碳酸钙的能力。从古吉拉特邦达科采集的富含粪便的土壤样本中共分离出8种细菌。其中，在尿素琼脂培养基上的初步筛选中，发现两株菌株DGDK-3和DGDK-4产生相当水平的脲酶。根据形态学和生理学测试，更具体地说，通过16S rRNA基因测序分析，这些细菌被鉴定为拟芽孢杆菌菌株DGDK-3和芽孢杆菌菌株。DGDK-4。菌株DGDK-3和DGDK-4的脲酶活性分别为25IU/ml和89IU/ml。此外，还测试了两种菌株对碳酸钙沉淀的功效。结果表明，两个分离物都能沉淀出显著水平的碳酸钙。目前的工作表明，产生脲酶的细菌可以用作生物水泥的裂缝密封剂和天然稳定剂。

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Isolation and Characterisation of Urease Producing Bacteria and their Capacity to Precipitate Calcium Carbonate

The current study was designed to isolate and characterize urease-producing bacteria and to assess their ability to precipitate calcium carbonate. Total eight bacteria were isolated from dung-rich soil samples collected from Dakor, Gujarat. Out of these, two bacterial strains designated as DGDK-3 and DGDK-4 were found to produce a considerable level of urease in the initial screening on the urea agar medium. Based on morphological and physiological tests and more specifically by 16S rRNA gene sequencing analysis, these bacteria were identified as Quasibacillus sp. Strain DGDK-3 and Bacillus sp. Strain DGDK-4. The strains DGDK-3 and DGDK-4 showed 25 IU/ml and 89 IU/ml urease activity, respectively. Also, the efficacy of both strains was tested for calcium carbonate precipitation. Results showed that both the isolates were competent to precipitate a significant level of calcium carbonate. The current work demonstrated that urease-producing bacteria can be utilised in bio-cementationas a crack sealing agent and as a natural stabilizing agent.

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来源期刊

Defence Life Science Journal Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

0.80

自引率

0.00%

发文量