增殖醋栗培养物在缓慢生长条件下的储存

IF 1 Q3 HORTICULTURE
D. Kucharska, T. Orlikowska, R. Maciorowski, M. Kunka, Angelika Niewiadomska-Wnuk
{"title":"增殖醋栗培养物在缓慢生长条件下的储存","authors":"D. Kucharska, T. Orlikowska, R. Maciorowski, M. Kunka, Angelika Niewiadomska-Wnuk","doi":"10.17221/142/2020-hortsci","DOIUrl":null,"url":null,"abstract":"Short storage of in vitro cultures under slow-growth conditions is included in the commercial large-scale micropropagation process. It is dictated by the organizational scheme that provides temporary stop multiplication of shoots for some months. To avoid subculturing to fresh media every 4 weeks, which is obligatory for gooseberry, they can be kept in conditions that protect them from ageing, by slowing down their metabolism. To develop a rational schedule of gooseberry micropropagation, two experiments were used to adopt a temperature and length of time for storage. The best results were obtained with storage conditions at 2 °C for two or four months for proliferating cultures. Under these conditions, the percentage of necrotic shoots was low (< 10%), and shoot proliferation in the subsequent passages was at a level similar to proliferation cultures incubated in the growth room and sub-cultured monthly. The rate of shoots > 1 cm was higher than in the control in the growth room. Storage at 4 °C increased the probability of necrotic shoots up to 80% and decreased the number of all shoots and shoots > 1 cm in subsequent passages.","PeriodicalId":13110,"journal":{"name":"Horticultural Science","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2021-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Storage of proliferating gooseberry cultures under slow growth conditions\",\"authors\":\"D. Kucharska, T. Orlikowska, R. Maciorowski, M. Kunka, Angelika Niewiadomska-Wnuk\",\"doi\":\"10.17221/142/2020-hortsci\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Short storage of in vitro cultures under slow-growth conditions is included in the commercial large-scale micropropagation process. It is dictated by the organizational scheme that provides temporary stop multiplication of shoots for some months. To avoid subculturing to fresh media every 4 weeks, which is obligatory for gooseberry, they can be kept in conditions that protect them from ageing, by slowing down their metabolism. To develop a rational schedule of gooseberry micropropagation, two experiments were used to adopt a temperature and length of time for storage. The best results were obtained with storage conditions at 2 °C for two or four months for proliferating cultures. Under these conditions, the percentage of necrotic shoots was low (< 10%), and shoot proliferation in the subsequent passages was at a level similar to proliferation cultures incubated in the growth room and sub-cultured monthly. The rate of shoots > 1 cm was higher than in the control in the growth room. Storage at 4 °C increased the probability of necrotic shoots up to 80% and decreased the number of all shoots and shoots > 1 cm in subsequent passages.\",\"PeriodicalId\":13110,\"journal\":{\"name\":\"Horticultural Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2021-01-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Horticultural Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.17221/142/2020-hortsci\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"HORTICULTURE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Horticultural Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.17221/142/2020-hortsci","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"HORTICULTURE","Score":null,"Total":0}
引用次数: 1

摘要

在缓慢生长条件下,体外培养物的短期储存包括在商业大规模微繁殖过程中。这是由组织方案决定的,该方案提供了几个月的临时停止芽的繁殖。为了避免每4周进行一次新鲜培养基的继代培养,醋栗可以通过减缓新陈代谢来保护它们免于老化。为了制定合理的醋栗微繁时间表,采用不同的贮藏温度和贮藏时间进行了两项试验。增殖培养物在2℃条件下保存2个月或4个月效果最好。在这些条件下,坏死芽的比例很低(< 10%),后续传代的芽增殖水平与生长室培养和每月继代培养的增殖培养水平相似。生长室内的芽苗生长速率高于对照。4°C贮藏使坏死芽的概率增加80%,随后传代中所有芽的数量减少,芽的直径为0 ~ 1 cm。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Storage of proliferating gooseberry cultures under slow growth conditions
Short storage of in vitro cultures under slow-growth conditions is included in the commercial large-scale micropropagation process. It is dictated by the organizational scheme that provides temporary stop multiplication of shoots for some months. To avoid subculturing to fresh media every 4 weeks, which is obligatory for gooseberry, they can be kept in conditions that protect them from ageing, by slowing down their metabolism. To develop a rational schedule of gooseberry micropropagation, two experiments were used to adopt a temperature and length of time for storage. The best results were obtained with storage conditions at 2 °C for two or four months for proliferating cultures. Under these conditions, the percentage of necrotic shoots was low (< 10%), and shoot proliferation in the subsequent passages was at a level similar to proliferation cultures incubated in the growth room and sub-cultured monthly. The rate of shoots > 1 cm was higher than in the control in the growth room. Storage at 4 °C increased the probability of necrotic shoots up to 80% and decreased the number of all shoots and shoots > 1 cm in subsequent passages.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Horticultural Science
Horticultural Science Horticulture-园艺
CiteScore
2.20
自引率
0.00%
发文量
23
审稿时长
>12 weeks
期刊介绍: The journal publishes results of basic and applied research from all areas of horticulture, fruit-growing, vegetable-growing, wine-making and viticulture, floriculture, ornamental gardening, garden and landscape architecture, concerning plants that are grown under the conditions of European temperate zone, or field plants that are considered as horticultural cultures. Original scientific papers, short communications and review articles are published in the journal. Papers are published in English (British spelling).
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信