加州家兔生长激素(GH)及其受体(GHR)核苷酸多态性与体重的相关性分析

IF 0.8 4区农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE

World Rabbit Science Pub Date : 2022-03-31 DOI:10.4995/wrs.2022.13127

D. Gencheva, K. Velikov, P. Veleva

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引用次数: 2

摘要

本研究旨在评价生长激素基因(GH)中的c.78C>T和生长激素受体基因(GHR)中的c.106C>G两种单核苷酸多态性(snp)基因型对生长35、70和90日龄加利福尼亚种兔个体体重(IBW)的影响。限制性内切片段显示，携带c.78C>T SNP的家兔中有74.8%为杂合，携带c.106C>G SNP的家兔中有52.3%为杂合，表明该加利福尼亚人群具有中等水平的遗传多样性。基于单基因方法的关联分析显示，生长激素基因的c.78C>T多态性对PT TT基因型有显著影响，且检测个体受显性效应影响显著。纯合c.106C . > . G . CC基因型个体与杂合CG基因型个体间存在显著差异。携带c.106C> g CC基因型的家兔IBW最高(2462.0±198.3 g)，且检测个体受加性效应影响显著。本研究共发现c.78C>T和c.106C >g snp的9个组合基因型，其中双倍型分析只涉及4个主要组(CT/CC、CC/CG、CT/CG和CT/GG)。CT/CC和CC/CG基因型组合个体之间、CC/CG和CT/GG基因型个体之间存在显著差异。然而，携带CT/CC基因型组合的家兔在90日龄时的体重最高(2447.2±213.8 g)。90日龄个体体重的决定系数最高(R2=10.8%)，表明基因型组合的影响很大。综上所述，GH基因的c.78C>T和GHR基因的c.106C >g可能是提高加州家兔生长性能的候选基因，在家兔育种中具有潜在的应用价值。

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Association analysis of nucleotide polymorphisms in growth hormone (GH) and its receptor (GHR) with body weight in Californian rabbits

The objective of the present study was to evaluate the influence of the genotypes of two single nucleotide polymorphisms (SNPs) – c.78C>T located in the growth hormone gene (GH) and c.106C>G in the growth hormone receptor gene (GHR) on individual body weight (IBW) during the growing period at 35, 70 and 90 d of age on a total of 107 weaned Californian breed rabbits. The restriction fragments obtained revealed that 74.8% of the rabbits carrying c.78C>T SNP and 52.3% of the rabbits carrying c.106C>G SNP were heterozygous, which indicated a moderate level of genetic diversity in this Californian population. Association analysis based on a single-gene approach revealed that c.78C>T polymorphism in the GH gene had a significant effect (P<0.05) on the weight at 70 and 90 d of age. The highest IBW (2530.4±66.6 g) was observed in rabbits carrying the c.78C>T TT genotype, and detected individuals were significantly affected by the dominance effect. Significant differences were observed between individuals with homozygous c.106C>G CC genotype and those with heterozygous CG genotype. The highest IBW (2462.0±198.3 g) was observed in rabbits carrying the c.106C>G CC genotype and detected individuals were significantly affected by the additive effect. A total of nine combined genotypes of c.78C>T and c.106C>G SNPs was found in the study, of which only four major groups (CT/CC, CC/CG, CT/CG, and CT/GG) were concerned in the diplotype analysis. Significant differences were observed between individuals with CT/CC and CC/CG genotype combinations, and between those with the CC/CG and CT/GG diplotypes. However, the highest IBW at 90 d of age (2447.2±213.8 g) was observed in rabbits carrying the CT/CC genotype combinations. The highest coefficient of determination found for individual body weight at 90 d of age (R2=10.8%) indicated a high effect of genotype combinations. In conclusion, the results obtained suggested that c.78C>T of GH gene and c.106C>G of GHR gene could be useful candidate genes to improve growth performance in Californian rabbits with potential application in rabbit breeding programmes.

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来源期刊

World Rabbit Science 农林科学-奶制品与动物科学

CiteScore

1.70

自引率

25.00%

发文量

审稿时长

>36 weeks

期刊介绍： World Rabbit Science is the official journal of the World Rabbit Science Association (WRSA). One of the main objectives of the WRSA is to encourage communication and collaboration among individuals and organisations associated with rabbit production and rabbit science in general. Subject areas include breeding, genetics, production, management, environment, health, nutrition, physiology, reproduction, behaviour, welfare, immunology, molecular biology, metabolism, processing and products. World Rabbit Science is the only international peer-reviewed journal included in the ISI Thomson list dedicated to publish original research in the field of rabbit science. Papers or reviews of the literature submitted to World Rabbit Science must not have been published previously in an international refereed scientific journal. Previous presentations at a scientific meeting, field day reports or similar documents can be published in World Rabbit Science, but they will be also subjected to the peer-review process. World Rabbit Science will publish papers of international relevance including original research articles, descriptions of novel techniques, contemporaryreviews and meta-analyses. Short communications will only accepted in special cases where, in the Editor''s judgement, the contents are exceptionally exciting, novel or timely. Proceedings of rabbit scientific meetings and conference reports will be considered for special issues. World Rabbit Science is published in English four times a year in a single volume. Authors may publish in World Rabbit Science regardless of the membership in the World Rabbit Science Association, even if joining the WRSA is encouraged. Views expressed in papers published in World Rabbit Science represent the opinion of the author(s) and do not necessarily reflect the official policy of the WRSA or the Editor-in-Chief.