水飞蓟素对MCF7细胞OCT4、NANOG和P53表达的调控作用

Q2 Pharmacology, Toxicology and Pharmaceutics
M. Hajizadeh, Faezeh Esmaeili Ranjbar, A. Abasi, M. Abbasifard, M. Mahmoodi, Mojgan Noroozi-Karimabad
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引用次数: 0

摘要

乳腺癌症是世界上第二常见的死亡原因,从水飞蓟中提取的天然化合物水飞蓟素已经显示出抗癌特性。在本研究中,水飞蓟素被用于治疗MCF7细胞和抑制干细胞多能性基因,以及细胞增殖。MCF7细胞在由各种水飞蓟素提取物浓度(10、100、500、1000、2000、3000、4000和5000µg/mL)组成的RPMI-1640培养基存在下培养24、48和72小时。采用MTT法测定化合物对细胞增殖的抑制作用。在确认细胞凋亡后,通过定量实时PCR测定OCT4、NANOG和P53表达的倍数变化。与对照相比,当使用不同浓度的水飞蓟素提取物24、48和72小时时,细胞活力存在显著差异(p值<0.05)。实时PCR分析表明,与未处理的对照细胞相比,OCT4和NANOG的表达下调,而P53的表达上调(p值<0.05)。根据这些发现,水飞蓟素对MCF7细胞系有影响,并通过调节OCT4、NANOG和P53通路介导物发挥作用。水飞蓟素可能引入该化合物作为一种有前景的抗MCF7的治疗化合物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Regulatory Effects of the Silymarin on Expression of OCT4, NANOG, and P53 in MCF7 Cell Lines
Breast cancer was known as the second most common cause of death in the world, natural sources compound derived from milk thistle called silymarin had already shown anticancer properties. In the present study, silymarin was used to treat MCF7 cells and inhibition of stem cell pluripotency genes, as well as cell proliferation. MCF7 cells were cultured in the presence of RPMI-1640 medium consisting of various silymarin extract concentrations (10, 100, 500, 1000, 2000, 3000, 4000, and 5000 µg/mL) for 24, 48, and 72 hours. The inhibitory effects of the compound on cellular proliferation were assessed via employing MTT assay techniques. Following confirming apoptosis, the fold changes of OCT4, NANOG and P53 expression were determined by quantitative Real-Time PCR. There was a significant difference (p value<0.05) in cell viability when various concentrations of silymarin extract were used for 24, 48, and 72 h in comparison to the control. Real-Time- PCR analysis indicated that the expression of OCT4 and NANOG was downregulated while P53 upregulated in compare to untreated control cells (p value <0.05). According to these findings, the silymarin effects on MCF7 cell line and act via modulating OCT4, NANOG, and P53 pathway mediators. Silymarin may introduce this compound as a promising therapeutic compound against MCF7.
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来源期刊
Current Bioactive Compounds
Current Bioactive Compounds Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)
CiteScore
1.90
自引率
0.00%
发文量
112
期刊介绍: The journal aims to provide comprehensive review articles on new bioactive compounds with proven activities in various biological screenings and pharmacological models with a special emphasis on stereoeselective synthesis. The aim is to provide a valuable information source of bioactive compounds synthesized or isolated, which can be used for further development of pharmaceuticals by industry and academia. The journal should prove to be essential reading for pharmacologists, natural product chemists and medicinal chemists who wish to be kept informed and up-to-date with the most important developments on new bioactive compounds of natural or synthetic origin, including their stereoeselective synthesis.
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