不同蛋黄和平衡时间的TRIS编码器检测西门塔尔精子质量

Yendraliza Yendraliza, A. Sitorus, Muhammad Rodiallah, Zumarni Zumarni
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引用次数: 0

摘要

摘要。本研究的目的是观察来自三种平衡时间变化的有蹄类动物的黄色三角卵巢癌症中西门塔尔牛精子的质量。精液是用两只西门塔尔奶牛制成的阴道保存的,这两只奶牛来自西苏门答腊省Payakumbuh的BIB Tuah Sakato。庇护所在早上举行,每周1次,为期10周。所使用的项目是一个双因素组设计(RAK)。第一个因素是使用的黄色鸡蛋(Puyuh、Chicken和Itik),第二个因素是平衡时间(2、3和4小时)。通过回归试验确定最佳点。解冻后测量的参数包括运动性、活力、异常、全质膜和恢复率。研究表明,使用黄色鸡蛋产生活力65.33%、活力67.7%、异常15%、MPU 50%和回收率87.11%,黄色大鼠蛋产生活力67%、活力72%、异常12%、MPU 54%和回收率89.5%,黄色仓鼠蛋产生活力65.00%、活力70.67%、异常13.33%,MPU为51.33%,回收率为86.67%。不同蛋黄和平衡时间的TRIS稀释液中西门塔尔牛精子的质量本研究的目的是在平衡时间变化的情况下,对三种家禽的蛋黄三稀释液中西门塔尔公牛精子的质量进行研究。使用人工阴道从来自西苏门答腊Payakumbuh的BIB Tuah Sakato的2头西门塔尔奶牛身上采集精液。精液在早上进行,每周一次,持续10周。实验采用双因素随机分组设计(RBD)。第一个因素是所用蛋黄的类型,第二个因素是平衡时间。通过回归确定最佳点。解冻后测量的参数包括运动性、活力、异常、完整质膜和恢复率。结果表明,使用鸡的活力为65.33%,活力为67.7%,异常率为15%,膜浆完整率为50%,回收率为87.11%鸭蛋黄的活力为67%,活力为72%,异常率为12%,膜浆完整率为54%,回收率为89.5%;其中蛋黄活力65%,活力70.67%,异常12%,膜浆完整性51.33%,回收率86.67%;结果表明,在平衡时间为2小时的tris稀释液中使用鸭蛋对公牛西门塔尔精子的效果要好于3和4小时。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Kualitas Spermatozoa Sapi Simmental pada Pengencer TRIS dengan Kuning Telur dan Waktu Equilibrasi yang Berbeda
ABSTRACT. Tujuan penelitian adalah untuk melihat kualitas spermatozoa sapi Simmental pada pengencer tris kuning telur yang berasal dari tiga jenis unggas dengan variasi waktu equilibrasi. Semen ditampung menggunakan vagina buatan dari 2 ekor sapi Simmental dari BIB Tuah Sakato, Payakumbuh, Sumatera Barat. Penampungan dilakukan pagi hari, 1 x seminggu selama 10 minggu. Rancangan yang digunakan adalah Rancangan Acak Kelompok (RAK) dua faktor. Faktor pertama Jenis kuning telur yang digunakan (Puyuh, Ayam, dan Itik) dan faktor kedua adalah waktu equilibrasi (2, 3 dan 4 jam). Titik optimum ditentukan dengan uji regresi. Parameter diukur setelah thawing meliputi motilitas, viabilitas, abnormalitas, membrane plasma utuh dan recovery rate. Hasil penelitian memperlihatkan bahwa penggunaan kuning telur asal ternak ayam menghasilkan motilitas 65.33%, viabilitas 67.7%, abnormalitas 15%, MPU 50% dan nilai recovery rate 87.11%, kuning telur itik menghasilkan motilitas 67%, viabilitas 72%, abnormalitas 12%, MPU 54%, dan recovery rate 89.5% dan kuning telur puyuh menghasilkan motilitas 65.00%, viabilitas 70.67%, abnormalitas 13.33%, MPU 51.33% dan nilai recovery rate 86.67%. Kesimpulan penggunaan telur itik dalam pengencer tris pada spermatozoa sapi Simmental dengan waktu equilibrasi 2 jam lebih baik dari waktu equilibrasi 3 dan 4 jam.  (The quality of Simmental cattle sperm in TRIS diluent with different egg yolks and equilibration times) ABSTRAK. Aim of this research was to conduct the quality of sperm Simmental bull in egg yolk tris diluent from three types of poultry with variations in the equilibration time. Semen is collected using an artificial vagina from 2 Simmental cows from BIB Tuah Sakato, Payakumbuh, West Sumatra. The semen is carried out in the morning, once a week for 10 weeks. The experiment was design with two factor Randomized Block Design (RBD). The first factor was the type of egg yolk used and the second factor was the equilibration time. The optimum point is determined by regression. Parameters measured after thawing included motility, viability, abnormalities, intact plasma membrane and recovery rate. The results showed that using of chicken in 65.33% motility, 67.7% viability, 15% abnormality, 50% integrity membrane plasma, and 87.11% recovery rate; duck egg yolk resulted in 67% motility, 72% viability, 12% abnormality, 54% integrity membrane plasma, and 89.5% recovery rate; quail egg yolk resulted 65% motility, 70.67% viability, 12% abnormality, 51.33% integrity membrane plasma, and 86.67% recovery rate; The conclusion is that the use of duck eggs in tris diluent with a equilibration time of 2 hours is better than 3 and 4 hours for bull Simmental spermatozoa.
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