{"title":"调整适体开关用于生物传感疟疾蛋白","authors":"Maxine Forder, M. McKeague","doi":"10.26443/msurj.v17i1.176","DOIUrl":null,"url":null,"abstract":"Malaria is a life-threatening disease caused by a protozoan transmitted through mosquito bites. Early diagnosis is essential to start treatment and prevent further transmission. However, current diagnostic methods are expensive, time consuming, and lack the portability required for efficient testing. Emerging methods that are faster and more portable include specialized biosensors called aptasensors. This study compared a range of complementary probes against malaria-binding aptamers to develop an aptamer-switch based platform. One candidate showed a promising increase in fluorescence upon incubating with a malaria target protein. This work has the potential to be incorporated into an aptasensor for rapid detection of malaria infections.","PeriodicalId":91927,"journal":{"name":"McGill Science undergraduate research journal : MSURJ","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Tuning Aptamer-Switching for Biosensing Malarial Proteins\",\"authors\":\"Maxine Forder, M. McKeague\",\"doi\":\"10.26443/msurj.v17i1.176\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Malaria is a life-threatening disease caused by a protozoan transmitted through mosquito bites. Early diagnosis is essential to start treatment and prevent further transmission. However, current diagnostic methods are expensive, time consuming, and lack the portability required for efficient testing. Emerging methods that are faster and more portable include specialized biosensors called aptasensors. This study compared a range of complementary probes against malaria-binding aptamers to develop an aptamer-switch based platform. One candidate showed a promising increase in fluorescence upon incubating with a malaria target protein. This work has the potential to be incorporated into an aptasensor for rapid detection of malaria infections.\",\"PeriodicalId\":91927,\"journal\":{\"name\":\"McGill Science undergraduate research journal : MSURJ\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-04-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"McGill Science undergraduate research journal : MSURJ\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.26443/msurj.v17i1.176\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"McGill Science undergraduate research journal : MSURJ","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.26443/msurj.v17i1.176","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Tuning Aptamer-Switching for Biosensing Malarial Proteins
Malaria is a life-threatening disease caused by a protozoan transmitted through mosquito bites. Early diagnosis is essential to start treatment and prevent further transmission. However, current diagnostic methods are expensive, time consuming, and lack the portability required for efficient testing. Emerging methods that are faster and more portable include specialized biosensors called aptasensors. This study compared a range of complementary probes against malaria-binding aptamers to develop an aptamer-switch based platform. One candidate showed a promising increase in fluorescence upon incubating with a malaria target protein. This work has the potential to be incorporated into an aptasensor for rapid detection of malaria infections.