根细菌遗传回路设计。

Q2 Agricultural and Biological Sciences
生物设计研究(英文) Pub Date : 2022-09-01 eCollection Date: 2022-01-01 DOI:10.34133/2022/9858049
Christopher M Dundas, José R Dinneny
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引用次数: 0

摘要

转基因植物在应对全球粮食安全和农业可持续性挑战方面具有巨大的前景。然而,基于植物的遗传电路的构建受到缺乏良好表征的遗传部件和电路设计规则的限制。相比之下,细菌合成生物学的进步已经产生了大量的传感器、致动器和其他工具,可以用来构建细菌电路。由于根定殖细菌(根细菌)对植物的健康和生长有着重要的影响,这些微生物中的遗传回路设计可以用来间接设计植物,加快设计-构建-测试-学习周期。在这里,我们概述了设计根际细菌回路的遗传部分和最佳实践,重点是可用于监测/控制根际和植物过程的传感器、致动器和底盘物种。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Genetic Circuit Design in Rhizobacteria.

Genetic Circuit Design in Rhizobacteria.

Genetic Circuit Design in Rhizobacteria.

Genetic Circuit Design in Rhizobacteria.

Genetically engineered plants hold enormous promise for tackling global food security and agricultural sustainability challenges. However, construction of plant-based genetic circuitry is constrained by a lack of well-characterized genetic parts and circuit design rules. In contrast, advances in bacterial synthetic biology have yielded a wealth of sensors, actuators, and other tools that can be used to build bacterial circuitry. As root-colonizing bacteria (rhizobacteria) exert substantial influence over plant health and growth, genetic circuit design in these microorganisms can be used to indirectly engineer plants and accelerate the design-build-test-learn cycle. Here, we outline genetic parts and best practices for designing rhizobacterial circuits, with an emphasis on sensors, actuators, and chassis species that can be used to monitor/control rhizosphere and plant processes.

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来源期刊
CiteScore
3.90
自引率
0.00%
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