根癌农杆菌:一种适于合成生物学的细菌。

Q2 Agricultural and Biological Sciences
生物设计研究(英文) Pub Date : 2020-05-26 eCollection Date: 2020-01-01 DOI:10.34133/2020/8189219
Mitchell G Thompson, William M Moore, Niklas F C Hummel, Allison N Pearson, Collin R Barnum, Henrik V Scheller, Patrick M Shih
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引用次数: 10

摘要

根癌农杆菌是植物生物技术中的一个重要工具,因为它具有将DNA转移到宿主植物基因组中的天然能力。根癌A.tumefaciens的遗传操作在提高许多植物物种和品种的转化效率方面取得了相当大的进展。此外,有压倒性的证据表明,调节根癌分枝杆菌毒力的各种介质的表达可以导致更成功的植物转化;因此,应用合成生物学实现细菌的靶向工程可能为推进植物生物技术提供新的机会。在这篇综述中,我们强调了根癌分枝杆菌和植物宿主中的工程目标,这些目标可以通过精确的遗传控制更有效地利用,在更广泛的宿主植物中产生高质量的转化事件。然后,我们进一步讨论了根癌A.和植物工程在植物转化方面的现状,并描述了未来的工作如何结合严格的合成生物学方法来定制用于植物转化的根癌A.菌株。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

<i>Agrobacterium tumefaciens</i>: A Bacterium Primed for Synthetic Biology.

Agrobacterium tumefaciens: A Bacterium Primed for Synthetic Biology.

Agrobacterium tumefaciens is an important tool in plant biotechnology due to its natural ability to transfer DNA into the genomes of host plants. Genetic manipulations of A. tumefaciens have yielded considerable advances in increasing transformational efficiency in a number of plant species and cultivars. Moreover, there is overwhelming evidence that modulating the expression of various mediators of A. tumefaciens virulence can lead to more successful plant transformation; thus, the application of synthetic biology to enable targeted engineering of the bacterium may enable new opportunities for advancing plant biotechnology. In this review, we highlight engineering targets in both A. tumefaciens and plant hosts that could be exploited more effectively through precision genetic control to generate high-quality transformation events in a wider range of host plants. We then further discuss the current state of A. tumefaciens and plant engineering with regard to plant transformation and describe how future work may incorporate a rigorous synthetic biology approach to tailor strains of A. tumefaciens used in plant transformation.

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来源期刊
CiteScore
3.90
自引率
0.00%
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