蚊子CRISPR-Cas9基因组编辑指南RNA的设计和验证。

Cold Spring Harbor protocols Pub Date : 2024-09-03 DOI:10.1101/pdb.top107688

Ivan Hok Yin Lo, Benjamin J Matthews

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引用次数: 0

摘要

CRISPR-Cas9已经彻底改变了传统和非传统模式生物的基因编辑。该工具为蚊子基础生物学的新机制研究以及基于CRISPR-Cas9的新型媒介控制策略的开发打开了大门，包括在种群中传播遗传元素的基因驱动。尽管CRISPR的特异性、灵活性和易于部署的前景是真实的，但其实施仍然需要对每个感兴趣的新物种以及给定物种内的每个基因组靶标进行经验优化。在这里，我们提供了设计和测试用于基于CRISPR的基因编辑工具的单引导RNA的概述。

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Design and Validation of Guide RNAs for CRISPR-Cas9 Genome Editing in Mosquitoes.

CRISPR-Cas9 has revolutionized gene editing for traditional and nontraditional model organisms alike. This tool has opened the door to new mechanistic studies of basic mosquito biology as well as the development of novel vector control strategies based on CRISPR-Cas9, including gene drives that spread genetic elements in the population. Although the promise of the specificity, flexibility, and ease of deployment CRISPR is real, its implementation still requires empirical optimization for each new species of interest, as well as to each genomic target within a given species. Here, we provide an overview of designing and testing single-guide RNAs for the use of CRISPR-based gene editing tools.

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来源期刊

Cold Spring Harbor protocols Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

3.00

自引率

0.00%

发文量

163

期刊介绍： Cold Spring Harbor Laboratory is renowned for its teaching of biomedical research techniques. For decades, participants in its celebrated, hands-on courses and users of its laboratory manuals have gained access to the most authoritative and reliable methods in molecular and cellular biology. Now that access has moved online. Cold Spring Harbor Protocols is an interdisciplinary journal providing a definitive source of research methods in cell, developmental and molecular biology, genetics, bioinformatics, protein science, computational biology, immunology, neuroscience and imaging. Each monthly issue details multiple essential methods—a mix of cutting-edge and well-established techniques.