{"title":"败血症和活性氧对收缩过程中骨骼肌间质氧压的影响。","authors":"Naoki Hitosugi, Kazuki Hotta, Yoshikazu Taketa, Ren Takamizawa, Yutaka Fujii, Ryo Ikegami, Hajime Tamiya, Tatsuro Inoue, Atsuhiro Tsubaki","doi":"10.1111/micc.12833","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Objective</h3>\n \n <p>This study aims to examine the effect of sepsis on the dynamics of skeletal muscle partial oxygen pressure during muscle contractions as well as the effect of reactive oxygen species (ROS) scavenger (ascorbic acid, Asc).</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Twenty-seven male Sprague–Dawley rats (2–3 months old) were randomly assigned to three groups; sham, cecal ligation and puncture (CLP), or CLP plus ascorbic acid treatment group (CLP + Asc). Electrical stimuli-induced muscle contractions and partial oxygen pressure measurements were performed at 3 h after CLP. The interstitial oxygen pressure (<i>P</i>O<sub>2</sub>is) in the spinotrapezius muscle was measured by the phosphorescence quenching method.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>The <i>P</i>O<sub>2</sub>is at rest was not different between the three groups. The <i>P</i>O<sub>2</sub>is decreased from rest to contraction in all groups. Compared to the sham, the time to decrease <i>P</i>O<sub>2</sub>is was significantly faster in CLP but not in CLP + Asc (<i>p</i> < .05). Compared to the sham, the <i>P</i>O<sub>2</sub>is during muscle contractions was significantly lower in both CLP and CLP + Asc (<i>p</i> < .05, respectively).</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>Our results suggest that CLP-induced sepsis accelerated the decay of <i>P</i>O<sub>2</sub>is at the onset of muscle contractions and maintained a low level of <i>P</i>O<sub>2</sub>is during muscle contractions.</p>\n </section>\n </div>","PeriodicalId":18459,"journal":{"name":"Microcirculation","volume":null,"pages":null},"PeriodicalIF":1.9000,"publicationDate":"2023-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The effect of sepsis and reactive oxygen species on skeletal muscle interstitial oxygen pressure during contractions\",\"authors\":\"Naoki Hitosugi, Kazuki Hotta, Yoshikazu Taketa, Ren Takamizawa, Yutaka Fujii, Ryo Ikegami, Hajime Tamiya, Tatsuro Inoue, Atsuhiro Tsubaki\",\"doi\":\"10.1111/micc.12833\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Objective</h3>\\n \\n <p>This study aims to examine the effect of sepsis on the dynamics of skeletal muscle partial oxygen pressure during muscle contractions as well as the effect of reactive oxygen species (ROS) scavenger (ascorbic acid, Asc).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>Twenty-seven male Sprague–Dawley rats (2–3 months old) were randomly assigned to three groups; sham, cecal ligation and puncture (CLP), or CLP plus ascorbic acid treatment group (CLP + Asc). Electrical stimuli-induced muscle contractions and partial oxygen pressure measurements were performed at 3 h after CLP. The interstitial oxygen pressure (<i>P</i>O<sub>2</sub>is) in the spinotrapezius muscle was measured by the phosphorescence quenching method.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>The <i>P</i>O<sub>2</sub>is at rest was not different between the three groups. The <i>P</i>O<sub>2</sub>is decreased from rest to contraction in all groups. Compared to the sham, the time to decrease <i>P</i>O<sub>2</sub>is was significantly faster in CLP but not in CLP + Asc (<i>p</i> < .05). Compared to the sham, the <i>P</i>O<sub>2</sub>is during muscle contractions was significantly lower in both CLP and CLP + Asc (<i>p</i> < .05, respectively).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>Our results suggest that CLP-induced sepsis accelerated the decay of <i>P</i>O<sub>2</sub>is at the onset of muscle contractions and maintained a low level of <i>P</i>O<sub>2</sub>is during muscle contractions.</p>\\n </section>\\n </div>\",\"PeriodicalId\":18459,\"journal\":{\"name\":\"Microcirculation\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2023-10-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microcirculation\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/micc.12833\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"HEMATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microcirculation","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/micc.12833","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"HEMATOLOGY","Score":null,"Total":0}
The effect of sepsis and reactive oxygen species on skeletal muscle interstitial oxygen pressure during contractions
Objective
This study aims to examine the effect of sepsis on the dynamics of skeletal muscle partial oxygen pressure during muscle contractions as well as the effect of reactive oxygen species (ROS) scavenger (ascorbic acid, Asc).
Methods
Twenty-seven male Sprague–Dawley rats (2–3 months old) were randomly assigned to three groups; sham, cecal ligation and puncture (CLP), or CLP plus ascorbic acid treatment group (CLP + Asc). Electrical stimuli-induced muscle contractions and partial oxygen pressure measurements were performed at 3 h after CLP. The interstitial oxygen pressure (PO2is) in the spinotrapezius muscle was measured by the phosphorescence quenching method.
Results
The PO2is at rest was not different between the three groups. The PO2is decreased from rest to contraction in all groups. Compared to the sham, the time to decrease PO2is was significantly faster in CLP but not in CLP + Asc (p < .05). Compared to the sham, the PO2is during muscle contractions was significantly lower in both CLP and CLP + Asc (p < .05, respectively).
Conclusions
Our results suggest that CLP-induced sepsis accelerated the decay of PO2is at the onset of muscle contractions and maintained a low level of PO2is during muscle contractions.
期刊介绍：
The journal features original contributions that are the result of investigations contributing significant new information relating to the vascular and lymphatic microcirculation addressed at the intact animal, organ, cellular, or molecular level. Papers describe applications of the methods of physiology, biophysics, bioengineering, genetics, cell biology, biochemistry, and molecular biology to problems in microcirculation.
Microcirculation also publishes state-of-the-art reviews that address frontier areas or new advances in technology in the fields of microcirculatory disease and function. Specific areas of interest include: Angiogenesis, growth and remodeling; Transport and exchange of gasses and solutes; Rheology and biorheology; Endothelial cell biology and metabolism; Interactions between endothelium, smooth muscle, parenchymal cells, leukocytes and platelets; Regulation of vasomotor tone; and Microvascular structures, imaging and morphometry. Papers also describe innovations in experimental techniques and instrumentation for studying all aspects of microcirculatory structure and function.
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