无标记竞争性时间分辨荧光适体传感器,用于使用Tb3+离子的ssDNA敏化荧光检测Pb2+。

Masoomeh Esmaelpourfarkhani, Mohammad Ramezani, Mona Alibolandi, Khalil Abnous, Seyed Mohammad Taghdisi
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引用次数: 0

摘要

铅(Pb2+)是毒性最大的重金属之一,其对环境的污染和严重破坏是全球关注的问题。因此,有必要开发有效的传感方法。本研究描述了一种设计用于检测Pb2+离子的无标记竞争性时间分辨荧光(TRF)适体传感器的新策略。该适体传感器的传感原理是Pb2+和Tb3+离子与富含鸟嘌呤/胸腺嘧啶的序列(铅适体)结合的竞争,以及该序列对Tb3+的敏化发光。所开发的TRF适体传感器显示出从2.5nM到150nM的良好线性检测范围和645pM的检测限(LOD)。此外,所提出的TRF适体传感器对Pb2+具有高选择性,它也已成功用于检测牛奶和人类血清样品中的这种离子。该TRF适体传感器由于其TRF特性,具有分析时间短、操作简单、成本低、无标记、超过生物样品背景荧光干扰等优点。在本文中,我们提出了一个富含Tb3+/鸟嘌呤/胸腺嘧啶的序列系统,通过应用合适的富含鸟嘌呤的序列来设计针对不同靶标的不同适体传感器。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Label-free competitive time-resolved fluorescent aptasensor for the detection of Pb2+ using ssDNA-sensitized fluorescence of Tb3+ ions.

Lead (Pb2+) is one of the most toxic heavy metals, and its environmental pollution and serious damages is a global concern. Therefore, it is necessary to develop effective sensing methods. This study describes a new strategy for the design of label-free competitive time-resolved fluorescent (TRF) aptasensor for detecting Pb2+ ions. The sensing principle of this aptasensor is the competition between Pb2+ and Tb3+ ions to bind to the guanine/thymine-rich sequence (lead aptamer) and the sensitized luminescence of Tb3+ by this sequence. The developed TRF aptasensor demonstrated a good linear detection range from 2.5 nM to 150 nM and a limit of detection (LOD) of 645 pM. In addition, the proposed TRF aptasensor has a high selectivity towards Pb2+, and it has also been successfully utilized to detect this ion in milk and human serum samples. This TRF aptasensor offers advantages such as short analysis time, simple operation, low cost, being label-free, and surpassing the interference of background fluorescence of biological samples due to its TRF characteristic. In this paper, we propose a Tb3+/guanine/thymine-rich sequence system for designing different aptasensors against diverse targets by applying a proper guanine-rich sequence.

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