从溶液X射线散射透视结构生物物理学。

IF 3 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Uri Raviv , Roi Asor , Asaf Shemesh , Avi Ginsburg , Tal Ben-Nun , Yaelle Schilt , Yehonatan Levartovsky , Israel Ringel
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引用次数: 1

摘要

结构生物物理学目前的挑战包括确定大型自组装复合物的结构,解析复杂结构的团簇结构及其质量分数,以及揭示导致从其亚基形成复杂结构的动力学途径和机制。现代同步加速器溶液X射线散射数据能够同时提供高空间和高时间的结构数据,以应对当前结构生物物理学的挑战。这些数据与晶体学、NMR和低温TEM数据是互补的。然而,对溶液散射数据的分析具有挑战性;因此,SAS门户中列出了许多不同的分析工具(http://smallangle.org/),已开发。在这篇综述中,我们首先简要总结了经典的X射线散射分析,深入了解了基本的结构和相互作用参数。然后,我们描述了最近的发展,将模拟、理论和先进的X射线散射建模相结合,为自组装复合物的结构、能量学和动力学提供了独特的见解。结构信息对于理解导致自组装超分子结构和计算结构精细化的潜在物理化学原理至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Insight into structural biophysics from solution X-ray scattering

The current challenges of structural biophysics include determining the structure of large self-assembled complexes, resolving the structure of ensembles of complex structures and their mass fraction, and unraveling the dynamic pathways and mechanisms leading to the formation of complex structures from their subunits. Modern synchrotron solution X-ray scattering data enable simultaneous high-spatial and high-temporal structural data required to address the current challenges of structural biophysics. These data are complementary to crystallography, NMR, and cryo-TEM data. However, the analysis of solution scattering data is challenging; hence many different analysis tools, listed in the SAS Portal (http://smallangle.org/), were developed. In this review, we start by briefly summarizing classical X-ray scattering analyses providing insight into fundamental structural and interaction parameters. We then describe recent developments, integrating simulations, theory, and advanced X-ray scattering modeling, providing unique insights into the structure, energetics, and dynamics of self-assembled complexes. The structural information is essential for understanding the underlying physical chemistry principles leading to self-assembled supramolecular architectures and computational structural refinement.

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来源期刊
Journal of structural biology
Journal of structural biology 生物-生化与分子生物学
CiteScore
6.30
自引率
3.30%
发文量
88
审稿时长
65 days
期刊介绍: Journal of Structural Biology (JSB) has an open access mirror journal, the Journal of Structural Biology: X (JSBX), sharing the same aims and scope, editorial team, submission system and rigorous peer review. Since both journals share the same editorial system, you may submit your manuscript via either journal homepage. You will be prompted during submission (and revision) to choose in which to publish your article. The editors and reviewers are not aware of the choice you made until the article has been published online. JSB and JSBX publish papers dealing with the structural analysis of living material at every level of organization by all methods that lead to an understanding of biological function in terms of molecular and supermolecular structure. Techniques covered include: • Light microscopy including confocal microscopy • All types of electron microscopy • X-ray diffraction • Nuclear magnetic resonance • Scanning force microscopy, scanning probe microscopy, and tunneling microscopy • Digital image processing • Computational insights into structure
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