通过合并CRISPR筛选的可扩展单细胞RNA图谱分析转录组动力学的关键调节因子。

IF 33.1 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Nature biotechnology Pub Date : 2023-09-25 DOI:10.1038/s41587-023-01948-9

Zihan Xu, Andras Sziraki, Jasper Lee, Wei Zhou, Junyue Cao

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引用次数: 0

摘要

我们提出了一种组合索引方法，扰动科学动力学，用于在单细胞水平上捕获数百种遗传扰动中的整个转录组、新生转录组和单引导RNA（sgRNA）身份。通过分析针对各种生物过程的CRISPR联合筛选，我们展示了RNA合成、加工和降解、miRNA生物发生和线粒体mRNA加工过程中的基因表达调控，系统地解码了RNA时间动力学的全基因组调控网络。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Dissecting key regulators of transcriptome kinetics through scalable single-cell RNA profiling of pooled CRISPR screens

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Dissecting key regulators of transcriptome kinetics through scalable single-cell RNA profiling of pooled CRISPR screens

We present a combinatorial indexing method, PerturbSci-Kinetics, for capturing whole transcriptomes, nascent transcriptomes and single guide RNA (sgRNA) identities across hundreds of genetic perturbations at the single-cell level. Profiling a pooled CRISPR screen targeting various biological processes, we show the gene expression regulation during RNA synthesis, processing and degradation, miRNA biogenesis and mitochondrial mRNA processing, systematically decoding the genome-wide regulatory network that underlies RNA temporal dynamics at scale. mRNA kinetics are described by combining single-cell combinatorial indexing with metabolic labeling and pooled CRISPR screens.

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来源期刊

Nature biotechnology 工程技术-生物工程与应用微生物

CiteScore

63.00

自引率

1.70%

发文量

382

审稿时长

3 months

期刊介绍： Nature Biotechnology is a monthly journal that focuses on the science and business of biotechnology. It covers a wide range of topics including technology/methodology advancements in the biological, biomedical, agricultural, and environmental sciences. The journal also explores the commercial, political, ethical, legal, and societal aspects of this research. The journal serves researchers by providing peer-reviewed research papers in the field of biotechnology. It also serves the business community by delivering news about research developments. This approach ensures that both the scientific and business communities are well-informed and able to stay up-to-date on the latest advancements and opportunities in the field. Some key areas of interest in which the journal actively seeks research papers include molecular engineering of nucleic acids and proteins, molecular therapy, large-scale biology, computational biology, regenerative medicine, imaging technology, analytical biotechnology, applied immunology, food and agricultural biotechnology, and environmental biotechnology. In summary, Nature Biotechnology is a comprehensive journal that covers both the scientific and business aspects of biotechnology. It strives to provide researchers with valuable research papers and news while also delivering important scientific advancements to the business community.