{"title":"使用非侵入性微核细胞仪测定铸造工人的DNA损伤。","authors":"Hakimeh Nazari Khuniqi , Yahya Rasoulzadeh , Yousef Mohammadian","doi":"10.1016/j.mrgentox.2023.503686","DOIUrl":null,"url":null,"abstract":"<div><p>Workers in the foundry industry are exposed to hazardous chemical agents such as metal fumes, gases, vapor of molten metal, and respirable dust and hazardous physical agents such as heat, noise, and electromagnetic fields. Co-exposures to hazardous physical and chemical agents in foundry workplaces may cause DNA damage in workers. This study aimed to evaluate DNA damage in foundry workers. Thirty-three exposed foundry workers as a exposure groups and 33 non-exposed individuals as a control groups participated in this study. Buccal micronucleus cytome (BMCyt assay) assay was used to assess DNA damage. Results showed that foundry workers were under exposure to hazardous chemical and physical agents such as metal fumes and noise. The percentage of micronucleus (MN) cells in exposure group (0.59 ± 0.93 %) were statistically higher than control group (0.23 ± 0.23 %) (P < 0.05) %). Also, the percentage of nuclear bud cells and binucleated cells in exposure group were statistically higher than control group (P < 0.05). The percentage of differentiated normal cells were significantly higher in the control group compared to the exposed group (P < 0.05). Foundry workers are at risk of DNA damage; therefore, prevention measures need to be implemented to reduce exposure to air pollutants in foundry workplaces.</p></div>","PeriodicalId":18799,"journal":{"name":"Mutation research. Genetic toxicology and environmental mutagenesis","volume":"891 ","pages":"Article 503686"},"PeriodicalIF":2.3000,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"DNA damage in foundry workers using non-invasive micronucleus cytome assay\",\"authors\":\"Hakimeh Nazari Khuniqi , Yahya Rasoulzadeh , Yousef Mohammadian\",\"doi\":\"10.1016/j.mrgentox.2023.503686\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Workers in the foundry industry are exposed to hazardous chemical agents such as metal fumes, gases, vapor of molten metal, and respirable dust and hazardous physical agents such as heat, noise, and electromagnetic fields. Co-exposures to hazardous physical and chemical agents in foundry workplaces may cause DNA damage in workers. This study aimed to evaluate DNA damage in foundry workers. Thirty-three exposed foundry workers as a exposure groups and 33 non-exposed individuals as a control groups participated in this study. Buccal micronucleus cytome (BMCyt assay) assay was used to assess DNA damage. Results showed that foundry workers were under exposure to hazardous chemical and physical agents such as metal fumes and noise. The percentage of micronucleus (MN) cells in exposure group (0.59 ± 0.93 %) were statistically higher than control group (0.23 ± 0.23 %) (P < 0.05) %). Also, the percentage of nuclear bud cells and binucleated cells in exposure group were statistically higher than control group (P < 0.05). The percentage of differentiated normal cells were significantly higher in the control group compared to the exposed group (P < 0.05). Foundry workers are at risk of DNA damage; therefore, prevention measures need to be implemented to reduce exposure to air pollutants in foundry workplaces.</p></div>\",\"PeriodicalId\":18799,\"journal\":{\"name\":\"Mutation research. Genetic toxicology and environmental mutagenesis\",\"volume\":\"891 \",\"pages\":\"Article 503686\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2023-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mutation research. Genetic toxicology and environmental mutagenesis\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1383571823001043\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mutation research. Genetic toxicology and environmental mutagenesis","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1383571823001043","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
DNA damage in foundry workers using non-invasive micronucleus cytome assay
Workers in the foundry industry are exposed to hazardous chemical agents such as metal fumes, gases, vapor of molten metal, and respirable dust and hazardous physical agents such as heat, noise, and electromagnetic fields. Co-exposures to hazardous physical and chemical agents in foundry workplaces may cause DNA damage in workers. This study aimed to evaluate DNA damage in foundry workers. Thirty-three exposed foundry workers as a exposure groups and 33 non-exposed individuals as a control groups participated in this study. Buccal micronucleus cytome (BMCyt assay) assay was used to assess DNA damage. Results showed that foundry workers were under exposure to hazardous chemical and physical agents such as metal fumes and noise. The percentage of micronucleus (MN) cells in exposure group (0.59 ± 0.93 %) were statistically higher than control group (0.23 ± 0.23 %) (P < 0.05) %). Also, the percentage of nuclear bud cells and binucleated cells in exposure group were statistically higher than control group (P < 0.05). The percentage of differentiated normal cells were significantly higher in the control group compared to the exposed group (P < 0.05). Foundry workers are at risk of DNA damage; therefore, prevention measures need to be implemented to reduce exposure to air pollutants in foundry workplaces.
期刊介绍：
Mutation Research - Genetic Toxicology and Environmental Mutagenesis (MRGTEM) publishes papers advancing knowledge in the field of genetic toxicology. Papers are welcomed in the following areas:
New developments in genotoxicity testing of chemical agents (e.g. improvements in methodology of assay systems and interpretation of results).
Alternatives to and refinement of the use of animals in genotoxicity testing.
Nano-genotoxicology, the study of genotoxicity hazards and risks related to novel man-made nanomaterials.
Studies of epigenetic changes in relation to genotoxic effects.
The use of structure-activity relationships in predicting genotoxic effects.
The isolation and chemical characterization of novel environmental mutagens.
The measurement of genotoxic effects in human populations, when accompanied by quantitative measurements of environmental or occupational exposures.
The application of novel technologies for assessing the hazard and risks associated with genotoxic substances (e.g. OMICS or other high-throughput approaches to genotoxicity testing).
MRGTEM is now accepting submissions for a new section of the journal: Current Topics in Genotoxicity Testing, that will be dedicated to the discussion of current issues relating to design, interpretation and strategic use of genotoxicity tests. This section is envisaged to include discussions relating to the development of new international testing guidelines, but also to wider topics in the field. The evaluation of contrasting or opposing viewpoints is welcomed as long as the presentation is in accordance with the journal''s aims, scope, and policies.
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