低H2O2浓度下离体新生大鼠胰岛过氧化物酶系统的表征。

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochimica et biophysica acta. Molecular basis of disease Pub Date : 2004-10-14 DOI:10.1016/j.bbadis.2004.06.005

Luiz F. Stoppiglia, Luiz F. Rezende, Fabiano Ferreira, Eliane Filiputti, Everardo M. Carneiro, Antonio C. Boschero

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引用次数: 0

摘要

糖尿病发病时B细胞的破坏与氧化应激有关。在这项工作中，我们试图进一步追踪胰岛内H2O2的命运，并确定它是通过酶(过氧化物酶)活性介导的，还是通过与任何蛋白质链上的硫醇的化学反应介导的。我们的结果表明，胰岛细胞在亲水性(细胞质)和疏水性区室(细胞器和细胞核)具有非常相似的过氧化物酶活性，与样品的过氧化氢酶含量无关。这种活性由牺牲硫醇和非血红素催化位点的Fe3+/Mn3+离子组成。在存在高浓度NADP*和RS*时，疏水组分清除O2-的能力增加，并且高度依赖于RSH。相反，亲水性组分表现出较低的rsh依赖性活性，其中O2-清除与附着在蛋白质分子上的金属Cu2+/Fe3+/Mn3+离子有关。

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Characterization of the peroxidase system at low H2O2 concentrations in isolated neonatal rat islets

B cell destruction during the onset of diabetes mellitus is associated with oxidative stress. In this work, we attempted to further trace the fate of H₂O₂ inside the pancreatic islets and determine whether it is mediated by enzymatic (peroxidase) activity or by chemical reaction with thiols from any protein chain. Our results suggest that the islet cells have a very similar peroxidase activity at the hydrophilic (cytoplasm) and hydrophobic compartments (organelles and nucleus), independent of the catalase content of the samples. This activity is composed of sacrificial thiols and by proteins with Fe³⁺/Mn³⁺ ions at non-heme catalytic sites. The capacity of the hydrophobic fraction to scavenge O₂⁻ was increased in the presence of high concentrations of NADP and RS and was highly dependent on RSH. On the contrary, the hydrophilic fraction exhibited a low RSH-dependent activity where the O₂⁻ scavenging is related to metal Cu²⁺/Fe³⁺/Mn³⁺ ions attached to the protein molecules.

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来源期刊

Biochimica et biophysica acta. Molecular basis of disease 生物-生化与分子生物学

CiteScore

12.30

自引率

0.00%

发文量

218

审稿时长

32 days

期刊介绍： BBA Molecular Basis of Disease addresses the biochemistry and molecular genetics of disease processes and models of human disease. This journal covers aspects of aging, cancer, metabolic-, neurological-, and immunological-based disease. Manuscripts focused on using animal models to elucidate biochemical and mechanistic insight in each of these conditions, are particularly encouraged. Manuscripts should emphasize the underlying mechanisms of disease pathways and provide novel contributions to the understanding and/or treatment of these disorders. Highly descriptive and method development submissions may be declined without full review. The submission of uninvited reviews to BBA - Molecular Basis of Disease is strongly discouraged, and any such uninvited review should be accompanied by a coverletter outlining the compelling reasons why the review should be considered.