蓝舌病病毒血清17型序列变异与中和作用相关。

William C Wilson, Kristen A Bernard, Babara A Israel, James O Mecham
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引用次数: 15

摘要

蓝舌病病毒(BTV)是一种昆虫传播的轨道病毒,对牛羊养殖业具有重要意义。由L2编码的VP2蛋白含有中和性表位。先前,对BTV血清型17 (BTV-17)原型菌株产生了一组中和单克隆抗体(mab),并确定了中和结构域由三个重叠的表位组成。中和后的BTV-17原型菌株与未中和的BTV-17 198菌株之间存在30多个氨基酸变化。在本研究中,我们对另外8株BTV-17的L2基因进行了测序,以确定先前特征差异的保守程度,这些菌株分别代表了BTV-17的中和和非中和组。对推导出的氨基酸序列进行比较,发现每组中91%(30/33)的变化是保守的。编码VP5的M5基因序列也被检测,因为这种表面蛋白也被证明影响中和作用。通过对VP5蛋白的分析,BTV-17中和组和未中和组之间没有一致的变化。最后,测定了5个MAb中和逃逸突变体的L2序列,以确定参与中和和可能的毒力的特定氨基酸。所有五个突变体都包含1-3个氨基酸变化,这些变化与先前描述的可变区域非常接近。这些氨基酸的变化可能定义了先前描述的重叠中和结构域的关键位点。这是首次描述在一个确定的地理区域内共同传播的两种具有明显中和特征的BT病毒种群。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Bluetongue virus serotype 17 sequence variation associated with neutralization.

Bluetongue virus (BTV) is an insect-transmitted orbivirus of importance to the cattle and sheep industry. The VP2 protein, encoded by L2, contains neutralizing epitopes. Previously, a panel of neutralizing monoclonal antibodies (MAbs) to the BTV serotype 17 (BTV-17) prototype strain was generated and it was determined that the neutralization domain consists of three overlapping epitopes. Over 30 amino acid changes were found between a neutralized BTV-17 prototype strain and a non-neutralized BTV-17 198 strain. In this study, the L2 genes from eight additional strains, representing both the neutralized and non-neutralized groups of BTV-17, were sequenced to determine the degree of conservation of the previously characterized differences. Comparison of the deduced amino acid sequences showed that 91% (30/33) of the previously noted changes were conserved within each group. The sequence of the M5 gene that encodes VP5 was also examined, since this surface protein has also been shown to affect neutralization. No consistent changes were noted between the neutralized and non-neutralized groups of BTV-17 by analysis of the VP5 protein. Finally, the L2 sequences of five MAb neutralization escape mutants were determined to identify specific amino acids involved in neutralization and perhaps virulence. All five mutants contained 1-3 amino acid changes that were in close proximity to a previously described variable region. These amino acid changes likely define critical sites in the overlapping neutralization domains previously described. This is the first description of two BT virus populations that have distinct neutralization characteristics co-circulating in a defined geographical region.

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