{"title":"直接和间接免疫荧光技术在肠道病毒鉴定及抗体滴定中的应用。","authors":"J L RIGGS, G C BROWN","doi":"10.3181/00379727-110-27664","DOIUrl":null,"url":null,"abstract":"Summary Tissue culture cells infected with various enteroviruses show specific immunofluorescence when stained directly with conjugated fractionated antiserum. The indirect method of fluorescent antibody determination is an even more satisfactory diagnostic technic since it makes possible the detection and titration of enterovirus antibodies actively acquired following clinical or subclinical infection or from vaccination.","PeriodicalId":20675,"journal":{"name":"Proceedings of the Society for Experimental Biology and Medicine","volume":" ","pages":"833-7"},"PeriodicalIF":0.0000,"publicationDate":"1962-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3181/00379727-110-27664","citationCount":"22","resultStr":"{\"title\":\"Application of direct and indirect immunofluorescence for identification of enteroviruses and titrating their antibodies.\",\"authors\":\"J L RIGGS, G C BROWN\",\"doi\":\"10.3181/00379727-110-27664\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Summary Tissue culture cells infected with various enteroviruses show specific immunofluorescence when stained directly with conjugated fractionated antiserum. The indirect method of fluorescent antibody determination is an even more satisfactory diagnostic technic since it makes possible the detection and titration of enterovirus antibodies actively acquired following clinical or subclinical infection or from vaccination.\",\"PeriodicalId\":20675,\"journal\":{\"name\":\"Proceedings of the Society for Experimental Biology and Medicine\",\"volume\":\" \",\"pages\":\"833-7\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1962-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.3181/00379727-110-27664\",\"citationCount\":\"22\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Proceedings of the Society for Experimental Biology and Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3181/00379727-110-27664\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of the Society for Experimental Biology and Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3181/00379727-110-27664","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Application of direct and indirect immunofluorescence for identification of enteroviruses and titrating their antibodies.
Summary Tissue culture cells infected with various enteroviruses show specific immunofluorescence when stained directly with conjugated fractionated antiserum. The indirect method of fluorescent antibody determination is an even more satisfactory diagnostic technic since it makes possible the detection and titration of enterovirus antibodies actively acquired following clinical or subclinical infection or from vaccination.