前列腺癌进展过程中肿瘤组织NO水平及酶活性的变化。

Q3 Medicine

Experimental oncology Pub Date : 2022-08-01 DOI:10.32471/exp-oncology.2312-8852.vol-44-no-2.17785

A P Burlaka, S V Virko, V M Grygorenko, O А Samoylenko, A V Verbinenko, V O Shlyakhovenko

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引用次数: 0

摘要

目的:探讨不同分期前列腺上皮内瘤变(PIN)和前列腺癌(PCa)患者组织标本和血液中性粒细胞中诱导no合成酶活性、总rna酶活性的变化。材料与方法:采用电子顺磁共振自旋阱技术测定不同分期PIN和PCa患者肿瘤组织及中性粒细胞NO水平。用酶谱法测定PIN和PCa患者肿瘤组织中RNase活性。结果:我们发现前列腺肿瘤组织中NO水平明显高于PIN，且随着疾病进展而升高。对PCa患者中性粒细胞NO水平的分析表明，其值不分散，不依赖于疾病的分期。前列腺癌患者中性粒细胞NO水平较健康供者明显增高。同时随着前列腺癌的进展，前列腺肿瘤组织中RNase活性逐渐降低。结论:随着前列腺癌的进展，诱导no合成酶和rna酶的活性发生显著变化。

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THE CHANGES OF NO LEVEL AND RNase ACTIVITY IN TUMOR TISSUE ACCOMPANYING THE PROGRESSION OF PROSTATE CANCER.

Aim: To assess the inducible NO-synthase activity and the total RNase activity in tissue samples and blood neutrophils of the patients with prostate intraepithelial neoplasia (PIN) and prostate cancer (PCa) of different stages.

Materials and methods: NO level was measured in tumor tissue and neutrophils of patients with PIN and PCa of different stages by electron paramagnetic resonance using the spin traps technology. RNase activity in tumor tissue of patients with PIN and PCa was measured by the method of zymography.

Results: We have found that NO levels in prostate tumor tissue were significantly higher than in the PIN and increased along with the disease progression. Analysis of NO level in neutrophils of the PCa patients demonstrated that the values were not dispersed and did not depend on the stage of disease. NO level in neutrophils of the PCa patients increased manifold as compared with that in healthy donors. At the same time, the RNase activity in the prostate tumor tissue gradually decreased with PCa progression.

Conclusion: Activities of inducible NO-synthase and RNases change significantly with progression of PCa.

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来源期刊

Experimental oncology Medicine-Oncology

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： The Experimental Oncology is an English-language journal that publishes review articles, original contributions, short communications, case reports and technical advances presenting new data in the field of experimental and fundamental oncology. Manuscripts should be written in English, contain original work, which has not been published or submitted for publication elsewhere. It also implies the transfer of the Copyright from the author to “Experimental Oncology”. No part of journal publications may be reproduced, stored in a retrieval system or transmitted in any form or by any means without the prior permission of the publisher.