辐照对肿瘤患者细胞因子产生的影响。

IF 2.3 4区 医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Amichay Meirovitz, Menachem Gross, Shani Cohen, Aron Popovtzer, Vivian Barak
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引用次数: 0

摘要

背景:放射治疗因影响细胞因子的分泌而被用于治疗癌症患者。细胞因子水平与疾病参数相关,可作为患者的生物标志物。我们的目的是探讨辐照对体外(使用健康供体淋巴细胞)和体内(使用辐照后头颈癌患者的血清水平)细胞因子产生的影响,并将其与粘膜炎严重程度/需要经皮内镜胃镜检查(PEG)管安装的相关性。方法:健康供体体外培养淋巴细胞在5 Gy或10 Gy照射下产生细胞因子。此外,对23例头颈部肿瘤患者的血液进行60-72G体内照射,评估其炎症因子(肿瘤坏死因子(TNF)α、白细胞介素(IL)-6、IL-8、IL-18)、抗炎细胞因子IL-10和一般标志物sIL-2R。放疗后,选择发生粘膜炎的患者进行PEG管安装治疗。细胞因子水平的变化被研究作为对治疗/PEG管安装反应的预测性生物标志物。采用elisa试剂盒检测细胞因子产生水平。结果:辐照降低了所有被测细胞因子的水平,最显著的是IL-6和IL-8,与辐照剂量成正比。在患者中,细胞因子水平的增加与粘膜炎的严重程度和可能需要安装PEG管相关。结论:辐照降低了健康淋巴细胞中所有细胞因子的水平,且呈剂量依赖性,尤其是IL-6和IL-8。这项研究显示炎症细胞因子、sIL-2R和辐射毒性的高水平和增加与PEG的需求之间存在相关性。放疗后细胞因子水平的降低预示不需要PEG。因此,我们的研究表明,细胞因子的变化是头颈癌患者的预测性生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of irradiation on cytokine production in cancer patients.

Background: Irradiation, which affects cytokine secretion, is used to treat cancer patients. Cytokine levels have correlations to disease parameters, serving as biomarkers for patients. We aim to explore the effect of irradiation on cytokine production both in vitro (using lymphocytes from healthy donors) and in vivo (using serum levels of head and neck cancer patients following irradiation) and correlating them to mucositis severity/need for percutaneous endoscopic gastroscopy (PEG) tube installation.

Methods: Cytokine production by cultured lymphocytes from healthy donors, in vitro, following irradiation of 5 or 10 Gy. In addition, blood from 23 patients with head and neck cancers, irradiated by 60-72G in vivo, were assessed for inflammatory cytokines (tumor necrosis factor (TNF)α, interleukin (IL)-6, IL-8, IL-18), the anti-inflammatory cytokine IL-10, and the general marker sIL-2R. Following radiation, selected patients who were developing mucositis were treated by PEG tube installation. Changes in cytokine levels were studied as predictive biomarkers of response to therapy/PEG tube installation. Cytokine production levels were measured using ELISAs kits.

Results: Irradiation decreased the levels of all tested cytokines, most notably IL-6 and IL-8, proportional to irradiation dose. In patients, increases in cytokine levels, correlated with mucositis severity and potentially the need for PEG tube installation.

Conclusions: Irradiation decreased the levels of all cytokines of healthy lymphocytes in a dose-dependent manner, especially those of IL-6 and IL-8. This study shows a correlation between high and increasing levels of inflammatory cytokines, sIL-2R, plus radiation toxicity and the need for PEG. The reduction of cytokine levels after radiotherapy predicts that PEG will not be required. Thus, our study shows that cytokine changes are predictive biomarkers in head and neck cancer patients.

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来源期刊
International Journal of Biological Markers
International Journal of Biological Markers 医学-生物工程与应用微生物
CiteScore
4.10
自引率
0.00%
发文量
43
期刊介绍: IJBM is an international, online only, peer-reviewed Journal, which publishes original research and critical reviews primarily focused on cancer biomarkers. IJBM targets advanced topics regarding the application of biomarkers in oncology and is dedicated to solid tumors in adult subjects. The clinical scenarios of interests are screening and early diagnosis of cancer, prognostic assessment, prediction of the response to and monitoring of treatment.
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