通过单分子观察定量评价DNA双链断裂(DSBs)。

Q3 Biochemistry, Genetics and Molecular Biology

Enzymes Pub Date : 2022-01-01 Epub Date: 2022-10-03 DOI:10.1016/bs.enz.2022.08.002

Kenichi Yoshikawa

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引用次数: 0

摘要

通过对单个dna的单分子观察方法，将表明对各种损伤源的双链断裂(DSBs)的可靠分析成为可能。在几十千克碱基对以上的单个DNA，其长度尺度在几μm以上，表明通过在溶液中加入合适的荧光染料，可以在荧光显微镜下看到其整个构象。描述了DSBs定量评价的各种实例，以及抗氧化剂保护作用的评价。

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Quantitative evaluation of DNA double-strand breaks (DSBs) through single-molecule observation.

By adapting the method of single molecular observation for individual DNAs, it will be shown that reliable analysis of double-strand breaks, DSBs, becomes possible for various kinds of damage sources. Single DNA above the size of several-tens kilo base-pairs exhibits the length scale above several μm, indicating that their whole conformation is visible with fluorescence microscopy by adding suitable fluoresce dye to the solution. Various examples of the quantitative evaluation on DSBs are described, together with the evaluation of the protective effects of anti-oxidants.

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来源期刊

Enzymes Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

4.30

自引率

0.00%

发文量