从 Momordica Charantia L. 中分离出的核糖体失活蛋白 MAP30 能诱导肝细胞癌细胞凋亡。

IF 2.5 4区 医学 Q3 ONCOLOGY
Yiping Zhou, Di Yang, Zihao Qiang, Yanfa Meng, Ruigang Li, Xiang Fan, Wei Zhao, Yao Meng
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引用次数: 0

摘要

背景:据报道,核糖体失活蛋白(RIPs)具有抗肿瘤和抗病毒活性。最近的一项专利 CN202011568116.7 开发了一种制备 30 kDa Momordica 抗艾滋病毒蛋白(MAP30)的新方法。MAP30 是一种 I 型 RIP,通过 N-糖苷酶活性杀死各种肿瘤细胞,并不可逆地抑制蛋白质合成:评估 MAP30 在诱导人肝癌 HCC-LM3 细胞凋亡中的潜在作用,并阐明 MAP30 的分子机制:采用 CCK-8 检测法评估 HCC-LM3 细胞的增殖情况。流式细胞术用于测量 HCC-LM3 细胞的周期、ROS 水平和凋亡。用 Western 印迹法测定蛋白质水平:结果:用 MAP30 处理人肝癌 HCCLM3 细胞可降低其存活率和增殖率,且呈剂量依赖性。PI 染色显示细胞周期停滞在 G0/G1 期。此外,MAP30 还能提高 HCC-LM3 细胞在 24 小时处理过程中的 ROS 水平。为了进一步证实 MAP30 在诱导细胞凋亡中的作用,我们采用免疫印迹法检测了 PARP 多(ADP-核糖)聚合酶(PARP-1)、Casepase3 和裂解-Caspase9 等细胞凋亡相关蛋白的变化。我们发现,在用MAP30处理的HCC-LM3细胞中,PARP-1和Caspase-3下调,而Cleaved-Caspase9上调:结论:这项研究表明,MAP30 有可能成为治疗人类肝细胞癌的新型药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Ribosome-inactivating Protein MAP30 Isolated from Momordica Charantia L. Induces Apoptosis in Hepatocellular Carcinoma Cells.

Background: Ribosome-inactivating proteins (RIPs) have been reported to exert antitumor and anti-virus activities. A recent patent CN202011568116.7 has developed a new method to prepare Momordica anti-HIV protein of 30 kDa (MAP30). MAP30 is a type I RIP, which kills various tumor cells through the N-glycosidase activity and irreversibly inhibits protein synthesis.

Objective: To assess the potential role of MAP30 in inducing apoptosis of human hepatocellular carcinoma HCC-LM3 cells and elucidate the molecular mechanism of MAP30.

Methods: CCK-8 assay was used to assess the proliferation of HCC-LM3 cells. Flow cytometry was used to measure the cycle, the level of ROS and apoptosis in HCC-LM3 cells. Western blots was used to measure protein levels.

Results: Treatment with MAP30 reduced survival and proliferation of human liver cancer HCCLM3 cells in a dose-dependent manner. PI staining showed cell cycle arrest in G0/G1 phase. Furthermore, MAP30 increased the level of ROS in HCC-LM3 cells in 24 h treatment. To further confirm the role of MAP30 in inducing cell apoptosis, immunoblotting was carried out to detect the change of apoptosis-related proteins including PARP poly (ADP-ribose) polymerase (PARP- 1), Casepase3 and Cleaved-Caspase9. We found that PARP-1 and Caspase-3 were downregulated, whereas Cleaved-Caspase9 was up-regulated in HCC-LM3 cells treated with MAP30.

Conclusion: This study indicated that MAP30 has the potential to be a novel therapeutic agent for human hepatocellular carcinoma.

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来源期刊
CiteScore
4.50
自引率
7.10%
发文量
55
审稿时长
3 months
期刊介绍: Aims & Scope Recent Patents on Anti-Cancer Drug Discovery publishes review and research articles that reflect or deal with studies in relation to a patent, application of reported patents in a study, discussion of comparison of results regarding application of a given patent, etc., and also guest edited thematic issues on recent patents in the field of anti-cancer drug discovery e.g. on novel bioactive compounds, analogs, targets & predictive biomarkers & drug efficacy biomarkers. The journal also publishes book reviews of eBooks and books on anti-cancer drug discovery. A selection of important and recent patents on anti-cancer drug discovery is also included in the journal. The journal is essential reading for all researchers involved in anti-cancer drug design and discovery. The journal also covers recent research (where patents have been registered) in fast emerging therapeutic areas/targets & therapeutic agents related to anti-cancer drug discovery.
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