Salma Ahi, Fatemeh Ebrahimi, Hassan Ali Abedi, Hossein Kargar Jahromi, Safar Zarei
{"title":"蚕茧水醇提取物对链脲佐菌素诱导的糖尿病雄性大鼠下丘脑-垂体-性腺轴的影响。","authors":"Salma Ahi, Fatemeh Ebrahimi, Hassan Ali Abedi, Hossein Kargar Jahromi, Safar Zarei","doi":"10.1155/2022/7916159","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Diabetes mellitus impairs the reproductive system by damaging the glands and changing their function and hormone secretions. Given the previous studies on medical properties of silk cocoon, the aim of this study was to investigate the effect of the hydroalcoholic extract of silk cocoon on pituitary-gonadal axis hormones and the testis changes in diabetic male rats.</p><p><strong>Methods: </strong>In this experimental study, 35 male rats were divided into 5 equal groups. Control (C), nontreated diabetic rats (DNT1), and experimental diabetic rats treated (DT1) with a silk cocoon extract at concentrations of 200, 400, and 800 mg/kg for 56 days. Diabetes was induced by an injection of streptozotocin. Blood sampling was performed by the tail and heart after fasting. Body weight, serum levels of glucose, prolactin, leptin, inhibin A, IGF-2, activin A, insulin, LH, testosterone, FSH, and GnRH were measured along with the testis weight and diameter as the outcome of the study. Data were analyzed by SPSS version 20.</p><p><strong>Results: </strong>Investigation of hormonal factors indicated that all diabetic groups had higher prolactin, inhibin A levels than those in C group and lower leptin, IGF-2, activin A, insulin, LH, testosterone, FSH, and GnRH levels than controls. Silk cocoon treatment significantly decreased prolactin and inhibin in comparison of DNT1 group. While there was a significant increase in leptin, IGF-2, activin A, insulin, LH, testosterone, FSH, and GnRH levels compared with DNT1 (<i>P</i> < 0.05). A significant decrease in both the testis weights and diameters was observed in diabetic male rats compared to controls (<i>P</i> < 0.05). While silk cocoon treatment improved gonadal weight, the diameter of tunica albuginea, and seminiferous tubules as long as increased in numbers of spermatocytes and Sertoli-Leydig cells. Spermatogonia, spermatocyte, spermatid, spermatozoid, Sertoli cells, and Leydig cell count were significantly lower in the DNT1 group in comparison with the control group, while all groups receiving the highest dose of SC800 mg/kg daily had a higher count of cells than the DNT1 group.</p><p><strong>Conclusion: </strong>It seems that silk cocoon treatment decreases the effects of diabetes on hypothalamic-pituitary-gonadal axis.</p>","PeriodicalId":46314,"journal":{"name":"Autoimmune Diseases","volume":" ","pages":"7916159"},"PeriodicalIF":1.7000,"publicationDate":"2022-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9605846/pdf/","citationCount":"2","resultStr":"{\"title\":\"The Effects of Hydroalcoholic Extract of Silk Cocoon on Hypothalamic-Pituitary -Gonadal Axis in Streptozotocin-Induced Diabetic Male Rats.\",\"authors\":\"Salma Ahi, Fatemeh Ebrahimi, Hassan Ali Abedi, Hossein Kargar Jahromi, Safar Zarei\",\"doi\":\"10.1155/2022/7916159\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Diabetes mellitus impairs the reproductive system by damaging the glands and changing their function and hormone secretions. Given the previous studies on medical properties of silk cocoon, the aim of this study was to investigate the effect of the hydroalcoholic extract of silk cocoon on pituitary-gonadal axis hormones and the testis changes in diabetic male rats.</p><p><strong>Methods: </strong>In this experimental study, 35 male rats were divided into 5 equal groups. Control (C), nontreated diabetic rats (DNT1), and experimental diabetic rats treated (DT1) with a silk cocoon extract at concentrations of 200, 400, and 800 mg/kg for 56 days. Diabetes was induced by an injection of streptozotocin. Blood sampling was performed by the tail and heart after fasting. Body weight, serum levels of glucose, prolactin, leptin, inhibin A, IGF-2, activin A, insulin, LH, testosterone, FSH, and GnRH were measured along with the testis weight and diameter as the outcome of the study. Data were analyzed by SPSS version 20.</p><p><strong>Results: </strong>Investigation of hormonal factors indicated that all diabetic groups had higher prolactin, inhibin A levels than those in C group and lower leptin, IGF-2, activin A, insulin, LH, testosterone, FSH, and GnRH levels than controls. Silk cocoon treatment significantly decreased prolactin and inhibin in comparison of DNT1 group. While there was a significant increase in leptin, IGF-2, activin A, insulin, LH, testosterone, FSH, and GnRH levels compared with DNT1 (<i>P</i> < 0.05). A significant decrease in both the testis weights and diameters was observed in diabetic male rats compared to controls (<i>P</i> < 0.05). While silk cocoon treatment improved gonadal weight, the diameter of tunica albuginea, and seminiferous tubules as long as increased in numbers of spermatocytes and Sertoli-Leydig cells. Spermatogonia, spermatocyte, spermatid, spermatozoid, Sertoli cells, and Leydig cell count were significantly lower in the DNT1 group in comparison with the control group, while all groups receiving the highest dose of SC800 mg/kg daily had a higher count of cells than the DNT1 group.</p><p><strong>Conclusion: </strong>It seems that silk cocoon treatment decreases the effects of diabetes on hypothalamic-pituitary-gonadal axis.</p>\",\"PeriodicalId\":46314,\"journal\":{\"name\":\"Autoimmune Diseases\",\"volume\":\" \",\"pages\":\"7916159\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2022-10-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9605846/pdf/\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Autoimmune Diseases\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1155/2022/7916159\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2022/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q4\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Autoimmune Diseases","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2022/7916159","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/1/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
引用次数: 2
摘要
背景:糖尿病通过损害腺体、改变其功能和激素分泌来损害生殖系统。鉴于前人对蚕茧药用特性的研究，本研究旨在探讨蚕茧水醇提取物对糖尿病雄性大鼠垂体-性腺轴激素及睾丸变化的影响。方法:将35只雄性大鼠随机分为5组。对照组(C)、未处理的糖尿病大鼠(DNT1)和实验糖尿病大鼠(DT1)分别给予浓度为200、400和800 mg/kg的蚕茧提取物56天。注射链脲佐菌素诱导糖尿病。禁食后经尾部和心脏采血。测量体重、血清葡萄糖、催乳素、瘦素、抑制素A、IGF-2、激活素A、胰岛素、LH、睾酮、FSH和GnRH水平以及睾丸重量和直径作为研究的结果。数据采用SPSS version 20进行分析。结果:激素因素调查显示，各糖尿病组催乳素、抑制素A水平均高于C组，瘦素、IGF-2、激活素A、胰岛素、LH、睾酮、FSH、GnRH水平均低于对照组。与DNT1组相比，蚕茧处理显著降低了泌乳素和抑制素。而与DNT1相比，瘦素、IGF-2、激活素a、胰岛素、LH、睾酮、FSH和GnRH水平显著升高(P < 0.05)。与对照组相比，糖尿病雄性大鼠睾丸重量和睾丸直径均显著降低(P < 0.05)。蚕茧处理提高了性腺的重量、白膜的直径和精小管的直径，并增加了精母细胞和支持间质细胞的数量。与对照组相比，DNT1组的精原细胞、精母细胞、精母细胞、类精子细胞、支持细胞和间质细胞计数显著降低，而SC800 mg/kg每日最高剂量组的细胞计数均高于DNT1组。结论:蚕茧治疗似乎可以降低糖尿病对下丘脑-垂体-性腺轴的影响。
The Effects of Hydroalcoholic Extract of Silk Cocoon on Hypothalamic-Pituitary -Gonadal Axis in Streptozotocin-Induced Diabetic Male Rats.
Background: Diabetes mellitus impairs the reproductive system by damaging the glands and changing their function and hormone secretions. Given the previous studies on medical properties of silk cocoon, the aim of this study was to investigate the effect of the hydroalcoholic extract of silk cocoon on pituitary-gonadal axis hormones and the testis changes in diabetic male rats.
Methods: In this experimental study, 35 male rats were divided into 5 equal groups. Control (C), nontreated diabetic rats (DNT1), and experimental diabetic rats treated (DT1) with a silk cocoon extract at concentrations of 200, 400, and 800 mg/kg for 56 days. Diabetes was induced by an injection of streptozotocin. Blood sampling was performed by the tail and heart after fasting. Body weight, serum levels of glucose, prolactin, leptin, inhibin A, IGF-2, activin A, insulin, LH, testosterone, FSH, and GnRH were measured along with the testis weight and diameter as the outcome of the study. Data were analyzed by SPSS version 20.
Results: Investigation of hormonal factors indicated that all diabetic groups had higher prolactin, inhibin A levels than those in C group and lower leptin, IGF-2, activin A, insulin, LH, testosterone, FSH, and GnRH levels than controls. Silk cocoon treatment significantly decreased prolactin and inhibin in comparison of DNT1 group. While there was a significant increase in leptin, IGF-2, activin A, insulin, LH, testosterone, FSH, and GnRH levels compared with DNT1 (P < 0.05). A significant decrease in both the testis weights and diameters was observed in diabetic male rats compared to controls (P < 0.05). While silk cocoon treatment improved gonadal weight, the diameter of tunica albuginea, and seminiferous tubules as long as increased in numbers of spermatocytes and Sertoli-Leydig cells. Spermatogonia, spermatocyte, spermatid, spermatozoid, Sertoli cells, and Leydig cell count were significantly lower in the DNT1 group in comparison with the control group, while all groups receiving the highest dose of SC800 mg/kg daily had a higher count of cells than the DNT1 group.
Conclusion: It seems that silk cocoon treatment decreases the effects of diabetes on hypothalamic-pituitary-gonadal axis.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
