Anmari Alvarez-Aleman, Margaret E Hunter, Thomas K Frazer, James A Powell, Eddy Garcia Alfonso, James D Austin
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引用次数: 1
摘要
古巴沿海水域是一小群濒临灭绝的西印度海牛的家园,这将受益于对该种群遗传变异的全面描述。我们对古巴海牛进行了首次遗传评估,以确定种群遗传结构的程度,并表征了群岛内各地区之间的中性遗传多样性。我们在18个微卫星位点对49只海牛进行了基因分型,对27个样本的1703个单核苷酸多态性(snp)进行了分型,并对59只海牛的线粒体控制区进行了测序。古巴海牛种群的核遗传多样性(微卫星HE = 0.44, SNP HE = 0.29)和线粒体遗传多样性(h = 0.068, π = 0.00025)较低,与随机交配的偏离程度适中(微卫星FIS = 0.12, SNP FIS = 0.10)。我们的研究结果表明,根据共同祖先和低但显著分化的证据,群岛西部经历了周期性的等位基因交换。关塔那摩湾东南部和群岛西部的海牛分化程度高于西南和西北海牛。在东南部观察到的遗传独特性支持了它作为自然资源管理的人口独立单位的认可,无论它是由于历史隔离还是由于距离隔离。利用微卫星和SNP数据集估算的区域有效种群规模较小(均为Ne
The first assessment of the genetic diversity and structure of the endangered West Indian manatee in Cuba.
The coastal waters of Cuba are home to a small, endangered population of West Indian manatee, which would benefit from a comprehensive characterization of the population's genetic variation. We conducted the first genetic assessment of Cuban manatees to determine the extent of the population's genetic structure and characterize the neutral genetic diversity among regions within the archipelago. We genotyped 49 manatees at 18 microsatellite loci, a subset of 27 samples on 1703 single nucleotide polymorphisms (SNPs), and sequenced 59 manatees at the mitochondrial control region. The Cuba manatee population had low nuclear (microsatellites HE = 0.44, and SNP HE = 0.29) and mitochondrial genetic diversity (h = 0.068 and π = 0.00025), and displayed moderate departures from random mating (microsatellite FIS = 0.12, SNP FIS = 0.10). Our results suggest that the western portion of the archipelago undergoes periodic exchange of alleles based on the evidence of shared ancestry and low but significant differentiation. The southeast Guantanamo Bay region and the western portion of the archipelago were more differentiated than southwest and northwest manatees. The genetic distinctiveness observed in the southeast supports its recognition as a demographically independent unit for natural resource management regardless of whether it is due to historical isolation or isolation by distance. Estimates of the regional effective population sizes, with the microsatellite and SNP datasets, were small (all Ne < 60). Subsequent analyses using additional samples could better examine how the observed structure is masking simple isolation by distance patterns or whether ecological or biogeographic forces shape genetic patterns.
期刊介绍:
Genetica publishes papers dealing with genetics, genomics, and evolution. Our journal covers novel advances in the fields of genomics, conservation genetics, genotype-phenotype interactions, evo-devo, population and quantitative genetics, and biodiversity. Genetica publishes original research articles addressing novel conceptual, experimental, and theoretical issues in these areas, whatever the taxon considered. Biomedical papers and papers on breeding animal and plant genetics are not within the scope of Genetica, unless framed in an evolutionary context. Recent advances in genetics, genomics and evolution are also published in thematic issues and synthesis papers published by experts in the field.