Hui Ni Huang, Zhu Ning Mo, Xiang Cheng Liao, Zi Ji Yang, Yue Rong Wei, Chun Li Zhu, Hai Lan Li
{"title":"10份ABO歧义血型样本的基因测序分析","authors":"Hui Ni Huang, Zhu Ning Mo, Xiang Cheng Liao, Zi Ji Yang, Yue Rong Wei, Chun Li Zhu, Hai Lan Li","doi":"10.19746/j.cnki.issn.1009-2137.2022.04.034","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Through analysis of ABO blood group gene typing technology, to assist in the identification of difficult clinical serological specimens.</p><p><strong>Methods: </strong>A total of 10 forwardreverse typing ambiguous samples were collected from January 2021 to August 2021 in our hospital.ABO genotypes were analysed by gene sequencing.</p><p><strong>Results: </strong>The genotypes of 10 ABO ambiguous blood group samples were A102/BW11, A102/BW12, O02/O02, A102/B303, A102/B101, BW11/O02, B101/O04, BW11/O01, BW11/O01, A101/O02, respectively. The genotype results of 6 cases was consistent with the serological phenotype, and the serological phenotype of 4 cases were different from the geno sequencing.</p><p><strong>Conclusion: </strong>ABO blood groups genotyping technology combined with serological typing can be used for accurate typing of ambiguous blood group, and better ensure the safety of blood transfusion.</p>","PeriodicalId":519535,"journal":{"name":"Zhongguo shi yan xue ye xue za zhi","volume":" ","pages":"1193-1197"},"PeriodicalIF":0.0000,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Gene Sequencing Analyses of 10 ABO Ambiguous Blood Group Samples].\",\"authors\":\"Hui Ni Huang, Zhu Ning Mo, Xiang Cheng Liao, Zi Ji Yang, Yue Rong Wei, Chun Li Zhu, Hai Lan Li\",\"doi\":\"10.19746/j.cnki.issn.1009-2137.2022.04.034\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>Through analysis of ABO blood group gene typing technology, to assist in the identification of difficult clinical serological specimens.</p><p><strong>Methods: </strong>A total of 10 forwardreverse typing ambiguous samples were collected from January 2021 to August 2021 in our hospital.ABO genotypes were analysed by gene sequencing.</p><p><strong>Results: </strong>The genotypes of 10 ABO ambiguous blood group samples were A102/BW11, A102/BW12, O02/O02, A102/B303, A102/B101, BW11/O02, B101/O04, BW11/O01, BW11/O01, A101/O02, respectively. The genotype results of 6 cases was consistent with the serological phenotype, and the serological phenotype of 4 cases were different from the geno sequencing.</p><p><strong>Conclusion: </strong>ABO blood groups genotyping technology combined with serological typing can be used for accurate typing of ambiguous blood group, and better ensure the safety of blood transfusion.</p>\",\"PeriodicalId\":519535,\"journal\":{\"name\":\"Zhongguo shi yan xue ye xue za zhi\",\"volume\":\" \",\"pages\":\"1193-1197\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zhongguo shi yan xue ye xue za zhi\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.19746/j.cnki.issn.1009-2137.2022.04.034\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zhongguo shi yan xue ye xue za zhi","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2022.04.034","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[Gene Sequencing Analyses of 10 ABO Ambiguous Blood Group Samples].
Objective: Through analysis of ABO blood group gene typing technology, to assist in the identification of difficult clinical serological specimens.
Methods: A total of 10 forwardreverse typing ambiguous samples were collected from January 2021 to August 2021 in our hospital.ABO genotypes were analysed by gene sequencing.
Results: The genotypes of 10 ABO ambiguous blood group samples were A102/BW11, A102/BW12, O02/O02, A102/B303, A102/B101, BW11/O02, B101/O04, BW11/O01, BW11/O01, A101/O02, respectively. The genotype results of 6 cases was consistent with the serological phenotype, and the serological phenotype of 4 cases were different from the geno sequencing.
Conclusion: ABO blood groups genotyping technology combined with serological typing can be used for accurate typing of ambiguous blood group, and better ensure the safety of blood transfusion.
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