小檗碱通过circ_ZNF608/miR-377-3p/COX2轴抑制子宫内膜癌的发展。

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
ACS Applied Electronic Materials Pub Date : 2022-11-01 Epub Date: 2022-07-24 DOI:10.1080/08916934.2021.2010050
Huan Liang, Yi Liu, Lian Fu, Ling Li, Nianjin Gong
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引用次数: 0

摘要

目的:子宫内膜癌是一种常见的女性恶性肿瘤。小檗碱(BBR)是一种具有抗肿瘤活性的生物碱,环状RNA (circRNAs)在癌症中得到了广泛的研究。然而,BBR是否通过调节环状RNA锌指蛋白608 (ZNF608)调控EC的发生尚不清楚。方法:采用不同浓度的BBR对子宫内膜癌细胞进行治疗。采用实时定量聚合酶链反应(qRT-PCR)检测circ_ZNF608、microRNA-377-3p (miR-377-3p)和环氧合酶2 (COX2)的表达。western blot检测COX2蛋白的表达。通过3-(4,5-二甲基噻唑-2-酰基)- 2,5 -二苯基溴化四唑(MTT)试验、胸腺嘧啶类似物5-乙基-2'-脱氧尿嘧啶(EdU)试验、菌落形成试验、transwell和流式细胞术验证circ_ZNF608对bbr处理的EC细胞的作用。采用活体异种移植肿瘤模型评价BBR和circ_ZNF608对肿瘤生长的影响。结果:小檗碱能抑制EC细胞的增殖和转移,促进凋亡,其作用与浓度有关。Circ_ZNF608和COX2在EC组织和细胞中异常升高,miR-377-3p水平逆转。过表达circ_ZNF608可恢复BBR对EC细胞的抑制作用。此外,circ_ZNF608通过抑制miR-377-3p的表达,恢复了BBR对EC细胞的抑制作用。同样,MiR-377-3p/COX2可以调节BBR下EC的肿瘤进展。最后,BBR在体内可以抑制子宫内膜癌的生长。结论:发现BBR通过circ_ZNF608/miR-377-3p/COX2轴抑制EC,对子宫内膜癌有帮助。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Berberine inhibits the development of endometrial cancer through circ_ZNF608/miR-377-3p/COX2 axis.

Objective: Endometrial carcinoma (EC) is a common malignant tumour in women. Berberin (BBR) is an alkaloid with anti-tumour activity, and circular RNA (circRNAs) has been extensively studied in cancers. However, whether BBR regulates the development of EC by regulating circular RNA zinc finger protein 608 (ZNF608) is unknown.

Methods: Different concentrations of BBR were used to treat endometrial cancer cells. A quantitative real-time polymerase chain reaction (qRT-PCR) was conducted to assess the expression of circ_ZNF608, microRNA-377-3p (miR-377-3p) and cyclooxygenase 2 (COX2). The expression of COX2 protein was detected by western blot. The effect of circ_ZNF608 in BBR-treated EC cells was verified by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, thymidine analog 5-ethynyl-2'-deoxyuridine (EdU) assay, colony formation assay, transwell, and flow cytometry. The effect of BBR and circ_ZNF608 on tumour growth was evaluated by xenograft tumour model in vivo.

Results: Berberine can inhibit the proliferation and metastasis of EC cells and promote apoptosis, which is related to the concentration. Circ_ZNF608 and COX2 were abnormally increased, while the levels of miR-377-3p were reversed in EC tissues and cells. Overexpression of circ_ZNF608 can restore the inhibitory effect of BBR on EC cells. In addition, circ_ZNF608 restored the inhibitory effect of BBR on EC cells by inhibiting the expression of miR-377-3p. Similarly, MiR-377-3p/COX2 can regulate the tumour progression of EC under BBR. Finally, BBR can inhibit the growth of endometrial carcinoma in vivo.

Conclusion: BBR was found to inhibit EC via the circ_ZNF608/miR-377-3p/COX2 axis, which is helpful in endometrial carcinoma.

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CiteScore
7.20
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4.30%
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