Tug1通过ERK12信号通路加重急性缺血性脑卒中后神经元损伤

IF 1.5 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Xiaojie Chen, Yuduan Xie, Chunling Liang, Dawei Yang, Xiaotao Li, Jie Yu
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引用次数: 3

摘要

约85%的脑卒中患者为缺血性脑卒中,缺血性脑卒中发病率高,预后困难。它已成为中老年人死亡的主要原因之一,严重威胁着人类的健康。本研究主要考虑lncRNA tug 1对ERK 12信号通路的作用,以增强急性缺血性脑卒中后神经元的损伤。本实验采用线栓法建立大脑中动脉闭塞(MCAO)模型。采用实时定量RT-CR法检测TG1、GAS5和SM22a基因在组织中的相对转录活性。免疫组化法检测SM22a蛋白在组织中的相对表达量。脑梗死24 h后,检测各组大鼠脑梗死侧皮层神经功能、脑梗死面积及ERK1/2蛋白表达水平。实验结果显示,生理盐水对照组和胃抑素A干扰组MCAO模型成功动物行为评分分别为1分25分、2分17分和3分18分。结果表明,lncRNA tug1可增强急性缺血性脑卒中后ERK12信号通路的神经元损伤。lncRNATUGl在OGD/RX后发挥重要作用,可加速细胞凋亡。lncRNATUGl表达受到抑制,细胞凋亡数量明显减少。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Tug1 Acts on ERK12 Signaling Pathway to Aggravate Neuronal Damage after Acute Ischemic Stroke.

Approximately 85% of stroke patients suffer from ischemic stroke, which has a high incidence and difficult prognosis. It has become one of the leading causes of death in middle-aged and elderly people and seriously threatens human health. This study mainly considers the role of lncRNA tug 1 on the ERK 12 signaling pathway to enhance neuronal damage after acute ischemic stroke. In the experiment, the middle cerebral artery occlusion (MCAO) model was constructed using the thread embolization method. The real-time quantitative RT-CR method was used to detect the relative transcriptional activity of TG1, GAS5 and SM22a genes in tissues. The relative expression level of SM22a protein in tissues was detected by the immune-histochemical method. Twenty-four hours after cerebral infarction, the nerve function, cerebral infarction area and ERK1/2 protein expression level of cerebral cortex on the side of cerebral infarction were detected in each group. The experimental results showed that the successful animal behavior scores of the MCAO model in the normal saline control group and Pepstatin A interference group were 1 point 25, 2 points 17 and 3 points 18. The results show that lncRNA tug1 can enhance the neuronal damage of the ERK12 signaling pathway after acute ischemic stroke. lncRNATUGl plays an important role after OGD/RX and can accelerate cell apoptosis. If the expression of lncRNATUGl is inhibited, the number of apoptosis is significantly reduced.

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来源期刊
Cellular and molecular biology
Cellular and molecular biology 生物-生化与分子生物学
CiteScore
1.60
自引率
12.50%
发文量
331
期刊介绍: Cellular and Molecular Biology publishes original articles, reviews, short communications, methods, meta-analysis notes, letters to editor and comments in the interdisciplinary science of Cellular and Molecular Biology linking and integrating molecular biology, biophysics, biochemistry, enzymology, physiology and biotechnology in a dynamic cell and tissue biology environment, applied to human, animals, plants tissues as well to microbial and viral cells. The journal Cellular and Molecular Biology is therefore open to intense interdisciplinary exchanges in medical, dental, veterinary, pharmacological, botanical and biological researches for the demonstration of these multiple links.
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