顺铂急性肾损伤中,Numb通过p53通路促进自噬。

IF 2.6 4区 医学 Q3 CELL BIOLOGY
Analytical Cellular Pathology Pub Date : 2022-06-27 eCollection Date: 2022-01-01 DOI:10.1155/2022/8213683
Ze Liu, Yong Li, Yongmei He, Junjie Wang
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引用次数: 3

摘要

急性肾损伤(AKI)是一个重要的公共卫生问题,其特征是涉及细胞凋亡和坏死的肾小管死亡。自噬在肾小管中迅速诱导,并与肾小管细胞稳态相关,与肾小管死亡有复杂的联系。麻蛋白是一种多功能蛋白,在顺铂致AKI肾小管死亡中发挥保护作用。然而,麻木对小管自噬的影响仍有待研究。本研究分析了顺铂诱导的敲除Numb的AKI小鼠自噬相关蛋白LC3和Beclin-1的表达。在体外顺铂诱导的小管损伤模型中,NRK-52E细胞Numb的下调也抑制了自噬的激活,同时p53蛋白水平降低。NRK-52E细胞过表达Numb可激活自噬,LC3和Beclin-1表达升高,p53蛋白水平升高。此外,麻木过表达后的自噬激活可被p53抑制剂聚氰菊酯-α抑制。这些数据表明,Numb促进顺铂诱导AKI中p53介导的小管自噬激活,因此可能为AKI的治疗提供重要靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Numb Promotes Autophagy through p53 Pathway in Acute Kidney Injury Induced by Cisplatin.

Numb Promotes Autophagy through p53 Pathway in Acute Kidney Injury Induced by Cisplatin.

Numb Promotes Autophagy through p53 Pathway in Acute Kidney Injury Induced by Cisplatin.

Numb Promotes Autophagy through p53 Pathway in Acute Kidney Injury Induced by Cisplatin.

Acute kidney injury (AKI) is an important public health concern and characterized as tubular death involved in apoptosis and necrosis. Autophagy is rapidly induced in tubules and associates with renal tubular cells homeostasis to have a complex link with tubular death in AKI. Numb is a multifunctional protein and exerts protective role in tubular death in AKI induced by Cisplatin. However, the effect of Numb on tubular autophagy remains to be investigated. In the present study, the protein expression of LC3 and Beclin-1 related to autophagy was analyzed in Cisplatin-induced AKI mice with knocking down Numb. In model of tubular injury induced by Cisplatin in vitro, downregulation of Numb in NRK-52E cells also inhibited the activation of autophagy accompanied with the decreased protein level of p53. Overexpression of Numb in NRK-52E cells activated autophagy with increased LC3 and Beclin-1 expression accompanied with increased protein level of p53. Moreover, autophagy activation following Numb overexpression was suppressed by p53 inhibitor pifithrin-α. These data indicate that Numb promotes p53-mediated activation of tubular autophagy in AKI induced by Cisplatin and therefore may provide important targets for the treatment of AKI.

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来源期刊
Analytical Cellular Pathology
Analytical Cellular Pathology ONCOLOGY-CELL BIOLOGY
CiteScore
4.90
自引率
3.10%
发文量
70
审稿时长
16 weeks
期刊介绍: Analytical Cellular Pathology is a peer-reviewed, Open Access journal that provides a forum for scientists, medical practitioners and pathologists working in the area of cellular pathology. The journal publishes original research articles, review articles, and clinical studies related to cytology, carcinogenesis, cell receptors, biomarkers, diagnostic pathology, immunopathology, and hematology.
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