Mutukuru Mayuri , Praveen T. Krishnamurthy , Thangavel Mahalingam Vijayakumar
{"title":"17-三氟甲基苯基三肽前列腺素F2α对人乳腺癌细胞系NK1受体的拮抗作用","authors":"Mutukuru Mayuri , Praveen T. Krishnamurthy , Thangavel Mahalingam Vijayakumar","doi":"10.1016/j.yexmp.2022.104817","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p><span>A growing number of genetic<span> and cancer biology<span> investigations have found that the tachykinin NK1 Receptor plays an important role in </span></span></span>cancer cell<span> proliferation and survival. In this study. The present study was designed to evaluate the inhibition of cell growth by 17-trifluoromethyl phenyl trinor prostaglandin<span> F2α with NK1 receptor in breast cancer cell lines.</span></span></p></div><div><h3>Materials and methods</h3><p><span><span>MDB-MB-468 and MCF-7 breast cancer cell lines were used in the experiment were blocked with PGF2a. Cell proliferation and </span>apoptosis<span> were analyzed to evaluate the cytotoxic effect. Cell cycle distribution, Caspase-3 enzyme activity<span>, Bad and Bax protein expression through flow cytometry and </span></span></span>molecular docking were carried out to analyze the NK1 receptor activity.</p></div><div><h3>Results</h3><p><span>We found that PGF2a has a high binding affinity<span><span> towards NK1 Receptor from molecular docking studies. It exerted cytotoxic and antiproliferative effects against MDB-MB-468 and MCF-7 breast cancer cell lines. Our data found that </span>treatment of cells with 17-TPGF2 resulted in cell death and showed that increased expression of Caspase-3, Bad, and Bax protein and further induces </span></span>G2 cell cycle arrest.</p></div><div><h3>Conclusion</h3><p>Overall this study investigates the NK1 receptor antagonistic effect of PGF2 against breast cancer cell lines. However, further studies are needed to better characterize the application of NK1 receptor inhibition in clinical cancer treatment and cytotoxicity effect.</p></div>","PeriodicalId":12176,"journal":{"name":"Experimental and molecular pathology","volume":"127 ","pages":"Article 104817"},"PeriodicalIF":2.8000,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"NK1 receptor antagonistic effect of 17-trifluoromethyl phenyl trinor prostaglandin F2α on the growth of human breast cancer cell line\",\"authors\":\"Mutukuru Mayuri , Praveen T. Krishnamurthy , Thangavel Mahalingam Vijayakumar\",\"doi\":\"10.1016/j.yexmp.2022.104817\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p><span>A growing number of genetic<span> and cancer biology<span> investigations have found that the tachykinin NK1 Receptor plays an important role in </span></span></span>cancer cell<span> proliferation and survival. In this study. The present study was designed to evaluate the inhibition of cell growth by 17-trifluoromethyl phenyl trinor prostaglandin<span> F2α with NK1 receptor in breast cancer cell lines.</span></span></p></div><div><h3>Materials and methods</h3><p><span><span>MDB-MB-468 and MCF-7 breast cancer cell lines were used in the experiment were blocked with PGF2a. Cell proliferation and </span>apoptosis<span> were analyzed to evaluate the cytotoxic effect. Cell cycle distribution, Caspase-3 enzyme activity<span>, Bad and Bax protein expression through flow cytometry and </span></span></span>molecular docking were carried out to analyze the NK1 receptor activity.</p></div><div><h3>Results</h3><p><span>We found that PGF2a has a high binding affinity<span><span> towards NK1 Receptor from molecular docking studies. It exerted cytotoxic and antiproliferative effects against MDB-MB-468 and MCF-7 breast cancer cell lines. Our data found that </span>treatment of cells with 17-TPGF2 resulted in cell death and showed that increased expression of Caspase-3, Bad, and Bax protein and further induces </span></span>G2 cell cycle arrest.</p></div><div><h3>Conclusion</h3><p>Overall this study investigates the NK1 receptor antagonistic effect of PGF2 against breast cancer cell lines. However, further studies are needed to better characterize the application of NK1 receptor inhibition in clinical cancer treatment and cytotoxicity effect.</p></div>\",\"PeriodicalId\":12176,\"journal\":{\"name\":\"Experimental and molecular pathology\",\"volume\":\"127 \",\"pages\":\"Article 104817\"},\"PeriodicalIF\":2.8000,\"publicationDate\":\"2022-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Experimental and molecular pathology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0014480022000806\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PATHOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental and molecular pathology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0014480022000806","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PATHOLOGY","Score":null,"Total":0}
NK1 receptor antagonistic effect of 17-trifluoromethyl phenyl trinor prostaglandin F2α on the growth of human breast cancer cell line
Background
A growing number of genetic and cancer biology investigations have found that the tachykinin NK1 Receptor plays an important role in cancer cell proliferation and survival. In this study. The present study was designed to evaluate the inhibition of cell growth by 17-trifluoromethyl phenyl trinor prostaglandin F2α with NK1 receptor in breast cancer cell lines.
Materials and methods
MDB-MB-468 and MCF-7 breast cancer cell lines were used in the experiment were blocked with PGF2a. Cell proliferation and apoptosis were analyzed to evaluate the cytotoxic effect. Cell cycle distribution, Caspase-3 enzyme activity, Bad and Bax protein expression through flow cytometry and molecular docking were carried out to analyze the NK1 receptor activity.
Results
We found that PGF2a has a high binding affinity towards NK1 Receptor from molecular docking studies. It exerted cytotoxic and antiproliferative effects against MDB-MB-468 and MCF-7 breast cancer cell lines. Our data found that treatment of cells with 17-TPGF2 resulted in cell death and showed that increased expression of Caspase-3, Bad, and Bax protein and further induces G2 cell cycle arrest.
Conclusion
Overall this study investigates the NK1 receptor antagonistic effect of PGF2 against breast cancer cell lines. However, further studies are needed to better characterize the application of NK1 receptor inhibition in clinical cancer treatment and cytotoxicity effect.
期刊介绍：
Under new editorial leadership, Experimental and Molecular Pathology presents original articles on disease processes in relation to structural and biochemical alterations in mammalian tissues and fluids and on the application of newer techniques of molecular biology to problems of pathology in humans and other animals. The journal also publishes selected interpretive synthesis reviews by bench level investigators working at the "cutting edge" of contemporary research in pathology. In addition, special thematic issues present original research reports that unravel some of Nature''s most jealously guarded secrets on the pathologic basis of disease.
Research Areas include: Stem cells; Neoangiogenesis; Molecular diagnostics; Polymerase chain reaction; In situ hybridization; DNA sequencing; Cell receptors; Carcinogenesis; Pathobiology of neoplasia; Complex infectious diseases; Transplantation; Cytokines; Flow cytomeric analysis; Inflammation; Cellular injury; Immunology and hypersensitivity; Athersclerosis.
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