Syahfitri Anita, Arif Rahman Sadjuri, Latri Rahmah, Herjuno Ari Nugroho, Mulyadi, Wahyu Trilaksono, Wiwit Ridhani, Nabila Safira, Hariman Bahtiar, Maharani, Amir Hamidy, Adriansjah Azhari
{"title":"印度尼西亚大斑蝶、岛斑蝶、尖斑蝶和紫斑蝶的毒液组成。","authors":"Syahfitri Anita, Arif Rahman Sadjuri, Latri Rahmah, Herjuno Ari Nugroho, Mulyadi, Wahyu Trilaksono, Wiwit Ridhani, Nabila Safira, Hariman Bahtiar, Maharani, Amir Hamidy, Adriansjah Azhari","doi":"10.1590/1678-9199-JVATITD-2021-0103","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Several studies have been published on the characterization of <i>Trimeresurus</i> venoms. However, there is still limited information concerning the venom composition of <i>Trimeresurus</i> species distributed throughout Indonesia, which contributes to significant snakebite envenomation cases. The present study describes a comparative on the composition of <i>T. albolabris, T. insularis, T. puniceus,</i> and <i>T. purpureomaculatus</i> venoms originated from Indonesia.</p><p><strong>Methods: </strong>Protein content in the venom of four <i>Trimeresurus</i> species was determined using Bradford assay, and the venom proteome was elucidated using one-dimension SDS PAGE nano-ESI- LCMS/MS shotgun proteomics.</p><p><strong>Results: </strong>The venom of <i>T. albolabris</i> contained the highest protein content of 11.1 mg/mL, followed by <i>T. puniceus</i>, <i>T. insularis</i> and <i>T. purpureomaculatus</i> venom with 10.7 mg/mL, 8.9 mg/mL and 5.54 mg/mL protein, respectively. In total, our venomic analysis identified 65 proteins belonging to 16 protein families in <i>T. purpureomaculatus</i>; 64 proteins belonging to 18 protein families in <i>T. albolabris</i>; 58 different proteins belonging to 14 protein families in <i>T. puniceus</i>; and 48 different proteins belonging to 14 protein familiesin <i>T. insularis.</i> Four major proteins identified in all venoms belonged to snake venom metalloproteinase, C-type lectin, snake venom serine protease, and phospholipase A2. There were 11 common proteins in all venoms, and <i>T. puniceus</i> venom has the highest number of unique proteins compared to the other three venoms. Cluster analysis of the proteins and venoms showed that <i>T. puniceus</i> venom has the most distinct venom composition.</p><p><strong>Conclusions: </strong>Overall, the results highlighted venom compositional variation of four <i>Trimeresurus</i> spp. from Indonesia. The venoms appear to be highly similar, comprising at least four protein families that correlate with venom's toxin properties and function. This study adds more information on venom variability among <i>Trimeresurus</i> species within the close geographic origin and may contribute to the development of optimum heterologous antivenom.</p>","PeriodicalId":17565,"journal":{"name":"Journal of Venomous Animals and Toxins Including Tropical Diseases","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2022-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9261747/pdf/","citationCount":"4","resultStr":"{\"title\":\"Venom composition of <i>Trimeresurus albolabris, T. insularis, T. puniceus</i> and <i>T. purpureomaculatus</i> from Indonesia.\",\"authors\":\"Syahfitri Anita, Arif Rahman Sadjuri, Latri Rahmah, Herjuno Ari Nugroho, Mulyadi, Wahyu Trilaksono, Wiwit Ridhani, Nabila Safira, Hariman Bahtiar, Maharani, Amir Hamidy, Adriansjah Azhari\",\"doi\":\"10.1590/1678-9199-JVATITD-2021-0103\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Several studies have been published on the characterization of <i>Trimeresurus</i> venoms. However, there is still limited information concerning the venom composition of <i>Trimeresurus</i> species distributed throughout Indonesia, which contributes to significant snakebite envenomation cases. The present study describes a comparative on the composition of <i>T. albolabris, T. insularis, T. puniceus,</i> and <i>T. purpureomaculatus</i> venoms originated from Indonesia.</p><p><strong>Methods: </strong>Protein content in the venom of four <i>Trimeresurus</i> species was determined using Bradford assay, and the venom proteome was elucidated using one-dimension SDS PAGE nano-ESI- LCMS/MS shotgun proteomics.</p><p><strong>Results: </strong>The venom of <i>T. albolabris</i> contained the highest protein content of 11.1 mg/mL, followed by <i>T. puniceus</i>, <i>T. insularis</i> and <i>T. purpureomaculatus</i> venom with 10.7 mg/mL, 8.9 mg/mL and 5.54 mg/mL protein, respectively. In total, our venomic analysis identified 65 proteins belonging to 16 protein families in <i>T. purpureomaculatus</i>; 64 proteins belonging to 18 protein families in <i>T. albolabris</i>; 58 different proteins belonging to 14 protein families in <i>T. puniceus</i>; and 48 different proteins belonging to 14 protein familiesin <i>T. insularis.</i> Four major proteins identified in all venoms belonged to snake venom metalloproteinase, C-type lectin, snake venom serine protease, and phospholipase A2. There were 11 common proteins in all venoms, and <i>T. puniceus</i> venom has the highest number of unique proteins compared to the other three venoms. Cluster analysis of the proteins and venoms showed that <i>T. puniceus</i> venom has the most distinct venom composition.</p><p><strong>Conclusions: </strong>Overall, the results highlighted venom compositional variation of four <i>Trimeresurus</i> spp. from Indonesia. The venoms appear to be highly similar, comprising at least four protein families that correlate with venom's toxin properties and function. This study adds more information on venom variability among <i>Trimeresurus</i> species within the close geographic origin and may contribute to the development of optimum heterologous antivenom.</p>\",\"PeriodicalId\":17565,\"journal\":{\"name\":\"Journal of Venomous Animals and Toxins Including Tropical Diseases\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2022-07-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9261747/pdf/\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Venomous Animals and Toxins Including Tropical Diseases\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1590/1678-9199-JVATITD-2021-0103\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2022/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q4\",\"JCRName\":\"TOXICOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Venomous Animals and Toxins Including Tropical Diseases","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1590/1678-9199-JVATITD-2021-0103","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/1/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"TOXICOLOGY","Score":null,"Total":0}
Venom composition of Trimeresurus albolabris, T. insularis, T. puniceus and T. purpureomaculatus from Indonesia.
Background: Several studies have been published on the characterization of Trimeresurus venoms. However, there is still limited information concerning the venom composition of Trimeresurus species distributed throughout Indonesia, which contributes to significant snakebite envenomation cases. The present study describes a comparative on the composition of T. albolabris, T. insularis, T. puniceus, and T. purpureomaculatus venoms originated from Indonesia.
Methods: Protein content in the venom of four Trimeresurus species was determined using Bradford assay, and the venom proteome was elucidated using one-dimension SDS PAGE nano-ESI- LCMS/MS shotgun proteomics.
Results: The venom of T. albolabris contained the highest protein content of 11.1 mg/mL, followed by T. puniceus, T. insularis and T. purpureomaculatus venom with 10.7 mg/mL, 8.9 mg/mL and 5.54 mg/mL protein, respectively. In total, our venomic analysis identified 65 proteins belonging to 16 protein families in T. purpureomaculatus; 64 proteins belonging to 18 protein families in T. albolabris; 58 different proteins belonging to 14 protein families in T. puniceus; and 48 different proteins belonging to 14 protein familiesin T. insularis. Four major proteins identified in all venoms belonged to snake venom metalloproteinase, C-type lectin, snake venom serine protease, and phospholipase A2. There were 11 common proteins in all venoms, and T. puniceus venom has the highest number of unique proteins compared to the other three venoms. Cluster analysis of the proteins and venoms showed that T. puniceus venom has the most distinct venom composition.
Conclusions: Overall, the results highlighted venom compositional variation of four Trimeresurus spp. from Indonesia. The venoms appear to be highly similar, comprising at least four protein families that correlate with venom's toxin properties and function. This study adds more information on venom variability among Trimeresurus species within the close geographic origin and may contribute to the development of optimum heterologous antivenom.
期刊介绍：
Journal of Venomous Animals and Toxins including Tropical Diseases (JVATiTD) is a non-commercial academic open access publication dedicated to research on all aspects of toxinology, venomous animals and tropical diseases. Its interdisciplinary content includes original scientific articles covering research on toxins derived from animals, plants and microorganisms. Topics of interest include, but are not limited to:systematics and morphology of venomous animals;physiology, biochemistry, pharmacology and immunology of toxins;epidemiology, clinical aspects and treatment of envenoming by different animals, plants and microorganisms;development and evaluation of antivenoms and toxin-derivative products;epidemiology, clinical aspects and treatment of tropical diseases (caused by virus, bacteria, algae, fungi and parasites) including the neglected tropical diseases (NTDs) defined by the World Health Organization.
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