施氮对不同坡度退化高寒草甸土壤真菌多样性的影响

Xiao-Xue Su, Xi-Lai Li, Cheng-Yi Li, Hua-Fang Sun
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引用次数: 0

摘要

本研究通过氮添加试验,分析外源氮添加对高寒草甸土壤真菌多样性的影响。所有实验均在三江源郭洛州两种不同坡度(缓坡和陡坡)的退化高寒草甸上进行,采用MiSeq PE250测序技术对土壤真菌ITS进行测序和分析。采用低氮(LN, 2 g·m-2)、中氮(MN, 5 g·m-2)和高氮(HN, 10 g·m-2) 3种氮素添加水平对退化草地不同坡度土壤真菌多样性的影响进行了对比分析。结果表明:①土壤中真菌的分布类群为子囊菌门、担子菌门、Mortierellomycota和Glomomycota,优势菌为子囊菌门。②Mortierella和Archaeorhizomyces是优势属,不同坡度和氮添加水平对土壤的响应没有差异。③共鉴定出赤霉素属(Gibberellum)、普氏菌属(Preussia)等95个属,两种不同坡度间差异显著(PPα和β多样性表明,不同坡度和施氮水平下土壤真菌群落结构稳定)。在不破坏土壤真菌群落结构的情况下,外源补氮显著提高了非优势真菌群落的相对丰度。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Effect of Nitrogen Addition on Soil Fungal Diversity in a Degraded Alpine Meadow at Different Slopes].

This study proposed nitrogen addition experiments to analyze the effects of exogenous nitrogen addition on soil fungal diversity in alpine meadow. All the experiments were performed in degraded alpine meadow with two different slopes (gentle slope and steep slope) in Guoluo Prefecture of the Sanjiangyuan Region, and the sequence and analysis of ITS of soil fungi were performed using MiSeq PE250 sequencing technology. Comparative analysis was carried out with three nitrogen addition levels on soil fungal diversity in degraded grassland with different slopes, which included low nitrogen (LN, 2 g·m-2), middle nitrogen (MN, 5 g·m-2), and high nitrogen (HN, 10 g·m-2). The results showed that:① the distribution groups of fungi in the soil were Ascomycota, Basidiomycota, Mortierellomycota, and Glomomycota, and the dominant bacteria was Ascomycota. ② The dominant genera were Mortierella and Archaeorhizomyces, and there were no differences in response to different slopes and nitrogen addition levels. ③ A total of 95 genera (Gibberellum, Preussia, etc.) were identified and significantly differed between two different slopes (P<0.05). ④ Bacteria with a relative abundance less than 1% had significant differences in nitrogen addition at different levels on the same slope (P<0.05). 5 In addition, the analyses of α and β diversities showed that soil fungal community structure was stable under different slopes and nitrogen addition levels. Exogenous nitrogen supplementation significantly improved the relative abundance of non-dominant fungal communities without destroying soil fungal community structure.

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