血小板群体中活化标志物表达的变化——血小板流式细胞术数据评价的新参数。

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
ACS Applied Bio Materials Pub Date : 2022-11-17 Epub Date: 2022-07-18 DOI:10.1080/09537104.2022.2078490
Nahreen Tynngård, Aseel Alshamari, Freja Månsson, Sofia Ramström
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引用次数: 1

摘要

流式细胞术研究单个细胞。血小板活化通常以表达标志物(阳性血小板)的血小板百分比和/或整个分析人群的平均/中位荧光强度(MFI)的形式报告。这些都没有考虑到单个血小板之间标记物表达的差异。这可以通过变异系数(CV)的数据得到。本研究探讨CV是否提供了血小板功能的额外信息。采用离心分离法(n = 13)和临时血小板单位法(n = 26)制备的血小板浓缩物(PCs),保存6-7天。自发和激动剂诱导的活化标志物(纤维蛋白原结合和p -选择素、LAMP-1和CD63的暴露)的表达以阳性血小板、MFI和CV的百分比进行研究。IPU pc自发表达p -选择素的阳性血小板百分比和MFI高于单采pc,相反,单采pc具有更高的激动剂诱导激活。单采pc的自发纤维蛋白原结合CV和p -选择素暴露CV较大,而IPU pc在激动剂刺激后p -选择素、LAMP-1和CD63的CV一般较大。我们的研究结果表明,通过提供血小板群体中活化反应变化的数据,CV在评估血小板活化时增加了额外的信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Variation in activation marker expression within the platelet population - a new parameter for evaluation of platelet flow cytometry data.

In flow cytometry, individual cells are investigated. Platelet activation is normally reported in form of percentage of platelets expressing the marker (positive platelets) and/or mean/median fluorescence intensity (MFI) for the entire analyzed population. None of these take into account the variance of the marker expression between individual platelets. This can be obtained as data on coefficient of variation (CV). This study explores if CV provides additional information regarding platelet function. Samples from platelet concentrates (PCs) prepared by apheresis- (n = 13) and interim platelet unit (IPU) technique (n = 26) and stored for 6-7 days were included and compared. Spontaneous- and agonist-induced expression of activation markers (fibrinogen binding and exposure of P-selectin, LAMP-1, and CD63) was investigated as percentage positive platelets, MFI and CV. Spontaneous expression of P-selectin as percentage positive platelets and MFI was higher for IPU PCs than apheresis PCs, which in contrast had higher agonist-induced activation. CV for spontaneous fibrinogen binding and P-selectin exposure was larger for apheresis PCs, while IPU PCs generally had larger CV for P-selectin, LAMP-1, and CD63 after agonist stimulation. Our findings show that CV adds additional information when assessing platelet activation by providing data on the variation in activation responses within the platelet population.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
期刊介绍: ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.
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