丝胶可减轻光周期诱导的成年小鼠睾丸和肾上腺甾体生成的异常改变。

Eman Hassan, Shahinaz Magdy, Amany Attaallah, Eman Gaber, Omnia Mansour, Rehab A Gomaa, Hala Odessy, Maria Augustyniak, Lamia M El-Samad, Abeer El Wakil
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引用次数: 1

摘要

背景:甾体生成是受气候变化影响的一系列酶促反应的复杂过程。动物对白昼长度的改变,即所谓的光周期,会产生生理上的变化。本研究旨在评价丝胶蛋白在减轻小鼠光周期紊乱引起的类固醇生成障碍中的作用。方法:根据光照周期将动物随机分为3组:对照组为标准的12Light:12Dark周期,短期光周期组为6Light:18Dark周期,长期光周期组为18Light:6Dark周期。短期组和长期组被细分为两个相等的亚组:安慰剂组和丝胶蛋白治疗组,从青春期前到成年期共5周,每周分别腹腔注射一次溶剂和每公斤体重1克丝胶蛋白。结果:测定了小鼠氧化应激参数及睾丸和肾上腺激素生成能力。五周后,光周期受损的安慰剂组表现出精子质量和数量的下降,睾酮、皮质酮、醛固酮、总抗氧化能力、黄嘌呤氧化酶和褪黑激素的减少。同时,在这些组中,肾上腺皮质的芳香化酶、丙二醛、胆固醇和类固醇生成因子-1 (SF-1)表达水平升高,组织学病变增强。接受丝胶治疗的小鼠参数与对照组相似。结论:丝胶蛋白可减轻光周期障碍对睾丸功能、性激素水平、精子数量和质量的影响。因此,丝胶蛋白是一种生物相容性蛋白,在异常光周期下的生物体中具有很好的应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Silk sericin alleviates aberrant photoperiod-induced alterations in testicular and adrenal steroidogenesis in adult mice.

Silk sericin alleviates aberrant photoperiod-induced alterations in testicular and adrenal steroidogenesis in adult mice.

Silk sericin alleviates aberrant photoperiod-induced alterations in testicular and adrenal steroidogenesis in adult mice.

Silk sericin alleviates aberrant photoperiod-induced alterations in testicular and adrenal steroidogenesis in adult mice.

Background: Steroidogenesis is a complex process of sequential enzymatic reactions affected by climate change. Animals respond to altered day length, the so-called photoperiod, with changes in physiology. The study aimed to an evaluation of sericin effect in alleviating steroidogenesis disorders induced by disturbed photoperiod in mice.

Methods: The animals were randomly divided into three groups according to the lighting cycle: a control group with a standard 12Light:12Dark cycle, a short-term photoperiod group with a 6Light:18Dark cycle, and a long-term photoperiod group with an 18Light:6Dark cycle. Both short and long-term groups were subdivided into two equal subgroups: The placebo and the sericin-treated subgroups received, for five weeks from prepubertal throughout adulthood, one intraperitoneal injection per week of the solvent and 1 g sericin/kg body weight, respectively.

Results: Selected oxidative stress parameters and testicular and adrenal steroidogenic capacities of adult mice were measured. After five weeks, the placebo group with impaired photoperiod showed a decrease in the quality and quantity of sperm and a reduction in testosterone, corticosterone, aldosterone, total antioxidant capacity, xanthine oxidase, and melatonin. At the same time, in these groups, there was an increase in the level of aromatase, malondialdehyde, cholesterol, and steroidogenic factor-1 (SF-1) expression in the adrenal cortex and an enhancement in histological lesions. Mice receiving sericin had parameters similar to the control group.

Conclusion: Our findings reveal that silk sericin can reduce the stress caused by photoperiod disorders regarding testicular function, sex hormone levels, and sperm quantity and quality. Thus, sericin is a biocompatible protein with a promising potential for its use in the case of organisms living under an abnormal photoperiod.

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