Role of GSL8 in low calcium tolerance in Arabidopsis thaliana.

Calcium (Ca) deficiency affects the yields and quality of agricultural products. Susceptibility to Ca deficiency varies among crops and cultivars; however, its genetic basis remains largely unknown. Genes required for low Ca tolerance in Arabidopsis thaliana have been identified. In this study, we identified a novel gene required for low Ca tolerance in A. thaliana. We isolated a mutant sensitive to low Ca concentrations and identified Glucan synthase-like (GSL) 8 as a gene responsible for low Ca tolerance. GSL8 is a paralog of the previously identified low Ca tolerance gene GSL10, which encodes β-1,3 glucan(callose) synthase. Under low Ca conditions, the shoot growth of gsl8 mutants were inhibited compared to wild-type plants. A grafting experiment indicated that the shoot, but not root, genotype was important for the shoot growth phenotype. The ectopic accumulation of callose under low Ca conditions was reduced in gsl8 mutants. We further investigated the interaction between GSL8 and GSL10 by testing the gsl8 gsl10 double mutant for sensitivity to low Ca concentrations. The double mutant exhibited a more severe phenotype than the single mutant under 0.3 mM Ca, indicating additive effects of GSL8 and GSL10 with respect to low Ca tolerance. These results establish that GSL genes are required for low Ca tolerance in A. thaliana.