酶联免疫吸附试验(ELISA)。

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2022-01-01 DOI:10.1007/978-1-0716-2376-3_10

Mahdis Sadat Tabatabaei, Marya Ahmed

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引用次数: 15

摘要

酶联免疫吸附试验(ELISA)是研究和临床中检测生物分子最特异性和最直接的方法之一。随着分析方法的进步，酶联免疫吸附试验(ELISA)不断优化以提高其灵敏度，目前已有不同类型的酶联免疫吸附试验(ELISA)可用于检测各种生物标志物。本章概述了ELISA的基本原理，讨论了ELISA检测的不同组成部分，并明确概述了不同类型ELISA检测的方案，包括直接、间接、夹心、竞争性和纳米颗粒型ELISA。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Enzyme-Linked Immunosorbent Assay (ELISA).

Enzyme-linked immunosorbent assay (ELISA) is one of the most specific and straightforward assays for detecting biomolecules in research and clinics. With advances in analytical methods, ELISA assay has been constantly optimized to improve its sensitivity, and different types of ELISA are now available to detect various biomarkers. This chapter provides an overall summary of the basic principle of ELISA, discusses different components of ELISA assay, and clearly outline protocols for different types of ELISA assays, including direct, indirect, sandwich, competitive, and nanoparticle-based ELISA.

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.