荧光原位杂交技术标记玉米减数分裂染色体。

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2013-01-01 DOI:10.1007/978-1-62703-333-6_4

Zhi Gao, Fangpu Han, Tatiana V Danilova, Jonathan C Lamb, Patrice S Albert, James A Birchler

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引用次数: 0

摘要

荧光原位杂交(FISH)可用于使用重复或单基因探针在最小目标尺寸以上可视化染色体特征。当应用于减数分裂时，核型补体的每条染色体都可以被识别，这有助于了解不同染色体在减数分裂过程中的相互关系。另一方面，早期减数分裂的粗线期的特征是染色体轻微但不强烈地凝聚，这使得对相邻特征的分析比体细胞中期染色体更详细。

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Labeling meiotic chromosomes in maize with fluorescence in situ hybridization.

Fluorescence in situ hybridization (FISH) can be used to visualize chromosomal features using repetitive or single gene probes above a minimum target size. When applied to meiosis, each chromosome of the karyotypic complement can be identified, which can facilitate an understanding of the interrelationship of different chromosomes during this process. On the other hand, the pachytene stage of early meiosis is characterized by slightly but not strongly condensed chromosomes that permit more detailed analyses of adjacent features than can be achieved with somatic metaphase chromosomes.

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.