植物病毒底物诱导BMP2的早期上调以促进骨的快速形成。

IF 1.4
Pongkwan Sitasuwan, L Andrew Lee, Peng Bo, Erin N Davis, Yuan Lin, Qian Wang
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引用次数: 47

摘要

许多纳米材料已经被开发出来,用于研究通过地形和化学线索对干细胞分化的影响,用于组织工程和再生医学。由于骨髓间充质干细胞(BMSCs)可快速诱导为成骨细胞,因此植物病毒作为细胞支持底物的使用一直受到特别关注。本研究探讨了烟草花叶病毒(TMV)在骨诱导中的作用及其对骨形态发生蛋白-2 (BMP2)的调控作用。我们观察到病毒底物上的细胞在24小时内立即聚集并形成骨样结节。在骨诱导8小时内,观察到TMV底物上细胞的BMP2基因和蛋白表达立即增加。此外,BMP2的表达高度局限于细胞聚集体内的细胞。这种增强的分化只发生在将TMV涂覆在固体载体上时,而不发生在将病毒加入培养基溶液时。综上所述,本研究的结果强调了基于病毒的纳米材料促进内源性BMP2产生的潜力,这可能是研究早期成骨细胞分化调控机制的一种独特方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A plant virus substrate induces early upregulation of BMP2 for rapid bone formation.

Many nanoscale materials have been developed to investigate the effects on stem cell differentiations via topographical and chemical cues for applications in tissue engineering and regenerative medicine. The use of plant viruses as cell supporting substrates has been of particular interest due to the rapid induction of bone marrow derived mesenchymal stem cells (BMSCs) towards osteogenic cells. In this study, the role of Tobacco mosaic virus (TMV) and its early effects on osteoinduction with particular emphasis on the regulation of bone morphogenetic protein-2 (BMP2) was examined. We observed that the cells on the virus substrate immediately aggregated and formed bone-like nodules within 24 hours. An immediate increase in BMP2 gene and protein expression for cells on the TMV substrate was observed within 8 hours of osteoinduction. Moreover, BMP2 expression was highly localized to cells within the cell aggregates. This enhanced differentiation only occurred when TMV was coated on a solid support but not upon adding the virus to the media solution. Taken together, the results from this study highlight the potential of virus-based nanomaterials to promote endogenous BMP2 production which may prove to be a unique approach to studying the regulatory mechanisms involved in early osteoblastic differentiation.

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