利用BAC-FISH技术定位二倍体龙葵粗线染色体的遗传连锁图谱。

Xiaomin Tang, Jan M de Boer, Herman J van Eck, Christian Bachem, Richard G F Visser, Hans de Jong
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引用次数: 53

摘要

建立了二倍体马铃薯(Solanum tuberosum)的细胞遗传学图谱,利用RHPOTKEY BAC文库中60个遗传锚定细菌人工染色体(BAC)克隆的多色荧光原位杂交(FISH)技术,在厚线素补体中鉴定了12个马铃薯二价体的臂。该诊断组选择的bac(每条染色体5个)与共色区杂交,并对应于超密集遗传图谱中定义明确的位点,通过这些探针可以建立新的详细可靠的粗线染色体核型。染色体大小已经估计从微观长度测量和从4',6-二氨基-2-苯基吲哚荧光DNA含量测量。在这两种方法中,1号染色体最大(100-115 Mb), 11号染色体最小(49-53 Mb)。5号染色体的百万碱基对与微米比率的详细测量结果显示,常染色质的平均值为1.07 Mb/mum,异染色质的平均值为3.67 Mb/mum。此外,我们的FISH结果有助于解决马铃薯遗传图谱中与染色体8和12相关的两个差异。最后,我们讨论了马铃薯细胞遗传图谱对马铃薯及相关茄科植物扩展FISH研究的意义,这将特别有利于马铃薯基因组测序计划。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Assignment of genetic linkage maps to diploid Solanum tuberosum pachytene chromosomes by BAC-FISH technology.

Assignment of genetic linkage maps to diploid Solanum tuberosum pachytene chromosomes by BAC-FISH technology.

Assignment of genetic linkage maps to diploid Solanum tuberosum pachytene chromosomes by BAC-FISH technology.

Assignment of genetic linkage maps to diploid Solanum tuberosum pachytene chromosomes by BAC-FISH technology.

A cytogenetic map has been developed for diploid potato (Solanum tuberosum), in which the arms of the 12 potato bivalents can be identified in pachytene complements using multicolor fluorescence in situ hybridization (FISH) with a set of 60 genetically anchored bacterial artificial chromosome (BAC) clones from the RHPOTKEY BAC library. This diagnostic set of selected BACs (five per chromosome) hybridizes to euchromatic regions and corresponds to well-defined loci in the ultradense genetic map, and with these probes a new detailed and reliable pachytene karyotype could be established. Chromosome size has been estimated both from microscopic length measurements and from 4',6-diamidino-2-phenylindole fluorescence-based DNA content measurements. In both approaches, chromosome 1 is the largest (100-115 Mb) and chromosome 11 the smallest (49-53 Mb). Detailed measurements of mega-base-pair to micrometer ratios have been obtained for chromosome 5, with average values of 1.07 Mb/mum for euchromatin and 3.67 Mb/mum for heterochromatin. In addition, our FISH results helped to solve two discrepancies in the potato genetic map related to chromosomes 8 and 12. Finally, we discuss the significance of the potato cytogenetic map for extended FISH studies in potato and related Solanaceae, which will be especially beneficial for the potato genome-sequencing project.

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