CREST，一种基于cas13的、坚固、公平、可扩展的检测方法(CREST)，用于在患者样本中检测SARS-CoV-2。

Current Protocols Pub Date : 2022-02-01 DOI:10.1002/cpz1.385

Zach Aralis, Jennifer N Rauch, Morgane Audouard, Eric Valois, Ryan S Lach, Sabrina Solley, Naomi J Baxter, Kenneth S Kosik, Maxwell Z Wilson, Diego Acosta-Alvear, Carolina Arias

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引用次数: 1

摘要

COVID-19大流行在世界范围内造成了毁灭性的人员伤亡。为控制COVID-19大流行制定有影响力的指南和措施，需要通过准确、可获取和可靠的SARS-CoV-2检测方法，对疑似病例及其接触者进行持续和广泛的检测。在这里，我们描述了一种基于crispr - cas13的检测SARS-CoV-2的方法。该检测方法被称为CREST(基于cas13的、坚固的、公平的、可扩展的检测)，具有特异性、敏感性和高度可及性。因此，CREST可能为SARS-CoV-2监测提供一种低成本和可靠的替代方案。©2022 Wiley期刊有限责任公司基本方案:使用实时PCR检测系统检测基于cas13的SARS-CoV-2遗传物质备用方案:使用荧光检测器检测基于cas13的SARS-CoV-2遗传物质支持方案1:LwaCas13a纯化支持方案2:合成靶点的体外转录。

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CREST, a Cas13-Based, Rugged, Equitable, Scalable Testing (CREST) for SARS-CoV-2 Detection in Patient Samples.

The COVID-19 pandemic has taken a devastating human toll worldwide. The development of impactful guidelines and measures for controlling the COVID-19 pandemic requires continuous and widespread testing of suspected cases and their contacts through accurate, accessible, and reliable methods for SARS-CoV-2 detection. Here we describe a CRISPR-Cas13-based method for the detection of SARS-CoV-2. The assay is called CREST (Cas13-based, rugged, equitable, scalable testing), and is specific, sensitive, and highly accessible. As such, CREST may provide a low-cost and dependable alternative for SARS-CoV-2 surveillance. © 2022 Wiley Periodicals LLC. Basic Protocol: Cas13-ased detection of SARS-CoV-2 genetic material using a real-time PCR detection system Alternate Protocol: Cas13-based detection of SARS-CoV-2 genetic material using a fluorescence viewer Support Protocol 1: LwaCas13a purification Support Protocol 2: In vitro transcription of synthetic targets.

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Current Protocols

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