ce掺杂生物活性玻璃/胶原/壳聚糖纳米复合支架对兔骨髓间充质干细胞源性成骨细胞形态和增殖的影响

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Hanan F Hammouda, Mohammad M Farag, Mervat M F El Deftar, M Abdel-Gabbar, Basant M Mohamed
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引用次数: 12

摘要

背景:含铈材料由于具有模拟催化活性,在生物医学领域有着广泛的应用。本研究旨在通过直接和间接方法将兔骨髓间充质干细胞(bmscs)首次传代至成骨系,深入评估基于ce掺杂纳米生物活性玻璃、胶原蛋白和壳聚糖的不同支架的生物相容性。支架中使用了1%的玻璃填料(30 wt. %),而玻璃中CeO2的百分比范围为0至10 mol. %。在成骨条件下维持的骨髓间充质干细胞,通过增殖试验监测细胞形态学变化和细胞增殖活性降低,每周两次使用MTT法直接接触细胞/支架,第7天使用台锥蓝和血细胞计细胞计数间接接触细胞/支架提取物。每天在显微镜下监测细胞行为生长、形态特征,并于周末将细胞与四种复合支架提取物在成骨培养基中孵育1周后进行细胞计数。结果:复合材料支架直接接触24 h后,所有支架的细胞增殖均大于50%,第7天细胞密度增加。生物活性玻璃纳米颗粒(样品CL/CH/C10)中CeO2含量最高的支架在第7天对细胞增殖的抑制作用最低(< 25%)。此外,间接细胞活力测试表明,四种复合支架提取物均未表现出对细胞的毒性作用(抑制值< 25%)。结论:在玻璃复合材料中添加CeO2可提高兔骨髓间充质干细胞向成骨谱系增殖复合支架的生物相容性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Effect of Ce-doped bioactive glass/collagen/chitosan nanocomposite scaffolds on the cell morphology and proliferation of rabbit's bone marrow mesenchymal stem cells-derived osteogenic cells.

Effect of Ce-doped bioactive glass/collagen/chitosan nanocomposite scaffolds on the cell morphology and proliferation of rabbit's bone marrow mesenchymal stem cells-derived osteogenic cells.

Effect of Ce-doped bioactive glass/collagen/chitosan nanocomposite scaffolds on the cell morphology and proliferation of rabbit's bone marrow mesenchymal stem cells-derived osteogenic cells.

Effect of Ce-doped bioactive glass/collagen/chitosan nanocomposite scaffolds on the cell morphology and proliferation of rabbit's bone marrow mesenchymal stem cells-derived osteogenic cells.

Background: Cerium-containing materials have wide applications in the biomedical field, because of the mimetic catalytic activities of cerium. The study aims to deeply estimate the biocompatibility of different scaffolds based on Ce-doped nanobioactive glass, collagen, and chitosan using the first passage of rabbit bone marrow mesenchymal stem cells (BM-MSCs) directed to osteogenic lineage by direct and indirect approach. One percentage of glass filler was used (30 wt. %) in the scaffold, while the percentage of CeO2 in the glass was ranged from 0 to 10 mol. %. Cytotoxicity was evaluated by monitoring of cell morphological changes and reduction in cell proliferation activity of BMMSCs maintained under osteogenic condition using proliferation assays, MTT assay for the direct contact of cells/scaffolds twice in a week, trypan blue and hemocytometer cell counting for indirect contact of cells/scaffolds extracts at day 7. Cell behaviors growth, morphology characteristics were monitored daily under a microscope and cell counting were conducted after 1 week of the incubation of the cells with the extracts of the four composite scaffolds in the osteogenic medium at the end of the week.

Results: Showed that at 24 h after direct contact with composite scaffold, all scaffolds showed proliferation of cells > 50% and increased in cell density on day 7. The scaffold of the highest percentage of CeO2 in bioactive glass nanoparticles (sample CL/CH/C10) showed the lowest inhibition of cell proliferation (< 25%) at day 7. Moreover, the indirect cell viability test showed that all extracts from the four composite scaffolds did not demonstrate a toxic effect on the cells (inhibition value < 25%).

Conclusion: The addition of CeO2 to the glass composition improved the biocompatibility of the composite scaffold for the proliferation of rabbit bone marrow mesenchymal stem cells directed to osteogenic lineage.

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