{"title":"TNF-α、IL-6和VDR基因对COVID-19发生风险和病程的调节作用试点研究。","authors":"Liliia Fishchuk, Zoia Rossokha, Valeriy Pokhylko, Yuliia Cherniavska, Svitlana Tsvirenko, Serhii Kovtun, Nataliia Medvedieva, Viktoriia Vershyhora, Nataliia Gorovenko","doi":"10.1515/dmpt-2021-0127","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>COVID-19 continues to range around the world and set morbidity and mortality antirecords. Determining the role of genetic factors in the development of COVID-19 may contribute to the understanding of the pathogenetic mechanisms that lead to the development of complications and fatalities in this disease. The aim of our study was to analyze the effect of <i>TNF-α</i> (rs1800629), <i>IL-6</i> (rs1800795) and <i>VDR</i> (rs731236 and rs1544410) genes variants on the development risk and the course of COVID-19 in intensive care patients.</p><p><strong>Methods: </strong>The study group included 31 patients with diagnosis \"viral COVID-19 pneumonia\". All patients underwent standard daily repeated clinical, instrumental and laboratory examinations. Determination of <i>IL-6</i>, <i>TNF-α</i>, and <i>VDR</i> genes variants was performed using the PCR-RFLP method.</p><p><strong>Results: </strong>It was found a significant increase in the rate of the CC genotype and C allele (38.7 vs. 12.0% and 0.6 vs. 0.4%, respectively) of the <i>IL-6</i> gene in all patients of the study in comparison with population frequencies. There was a significantly higher rate of heterozygous genotypes TC and GA of the <i>VDR</i> gene in group of died patients. The rs1800629 variant of the <i>TNF-α</i> gene is associated with the need for respiratory support and its longer duration in patients with COVID-19.</p><p><strong>Conclusions: </strong>The obtained results support a hypothesis about the influence of variants of <i>IL-6</i>, <i>TNF-α</i> and <i>VDR</i> genes on severity of COVID-19. However, in order to draw definite conclusions, further multifaceted research in this area are need.</p>","PeriodicalId":11332,"journal":{"name":"Drug metabolism and personalized therapy","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2021-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"11","resultStr":"{\"title\":\"Modifying effects of <i>TNF-α</i>, <i>IL-6</i> and <i>VDR</i> genes on the development risk and the course of COVID-19. Pilot study.\",\"authors\":\"Liliia Fishchuk, Zoia Rossokha, Valeriy Pokhylko, Yuliia Cherniavska, Svitlana Tsvirenko, Serhii Kovtun, Nataliia Medvedieva, Viktoriia Vershyhora, Nataliia Gorovenko\",\"doi\":\"10.1515/dmpt-2021-0127\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>COVID-19 continues to range around the world and set morbidity and mortality antirecords. Determining the role of genetic factors in the development of COVID-19 may contribute to the understanding of the pathogenetic mechanisms that lead to the development of complications and fatalities in this disease. The aim of our study was to analyze the effect of <i>TNF-α</i> (rs1800629), <i>IL-6</i> (rs1800795) and <i>VDR</i> (rs731236 and rs1544410) genes variants on the development risk and the course of COVID-19 in intensive care patients.</p><p><strong>Methods: </strong>The study group included 31 patients with diagnosis \\\"viral COVID-19 pneumonia\\\". All patients underwent standard daily repeated clinical, instrumental and laboratory examinations. Determination of <i>IL-6</i>, <i>TNF-α</i>, and <i>VDR</i> genes variants was performed using the PCR-RFLP method.</p><p><strong>Results: </strong>It was found a significant increase in the rate of the CC genotype and C allele (38.7 vs. 12.0% and 0.6 vs. 0.4%, respectively) of the <i>IL-6</i> gene in all patients of the study in comparison with population frequencies. There was a significantly higher rate of heterozygous genotypes TC and GA of the <i>VDR</i> gene in group of died patients. The rs1800629 variant of the <i>TNF-α</i> gene is associated with the need for respiratory support and its longer duration in patients with COVID-19.</p><p><strong>Conclusions: </strong>The obtained results support a hypothesis about the influence of variants of <i>IL-6</i>, <i>TNF-α</i> and <i>VDR</i> genes on severity of COVID-19. However, in order to draw definite conclusions, further multifaceted research in this area are need.</p>\",\"PeriodicalId\":11332,\"journal\":{\"name\":\"Drug metabolism and personalized therapy\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-12-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"11\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Drug metabolism and personalized therapy\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1515/dmpt-2021-0127\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Pharmacology, Toxicology and Pharmaceutics\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Drug metabolism and personalized therapy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1515/dmpt-2021-0127","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Pharmacology, Toxicology and Pharmaceutics","Score":null,"Total":0}
Modifying effects of TNF-α, IL-6 and VDR genes on the development risk and the course of COVID-19. Pilot study.
Objectives: COVID-19 continues to range around the world and set morbidity and mortality antirecords. Determining the role of genetic factors in the development of COVID-19 may contribute to the understanding of the pathogenetic mechanisms that lead to the development of complications and fatalities in this disease. The aim of our study was to analyze the effect of TNF-α (rs1800629), IL-6 (rs1800795) and VDR (rs731236 and rs1544410) genes variants on the development risk and the course of COVID-19 in intensive care patients.
Methods: The study group included 31 patients with diagnosis "viral COVID-19 pneumonia". All patients underwent standard daily repeated clinical, instrumental and laboratory examinations. Determination of IL-6, TNF-α, and VDR genes variants was performed using the PCR-RFLP method.
Results: It was found a significant increase in the rate of the CC genotype and C allele (38.7 vs. 12.0% and 0.6 vs. 0.4%, respectively) of the IL-6 gene in all patients of the study in comparison with population frequencies. There was a significantly higher rate of heterozygous genotypes TC and GA of the VDR gene in group of died patients. The rs1800629 variant of the TNF-α gene is associated with the need for respiratory support and its longer duration in patients with COVID-19.
Conclusions: The obtained results support a hypothesis about the influence of variants of IL-6, TNF-α and VDR genes on severity of COVID-19. However, in order to draw definite conclusions, further multifaceted research in this area are need.
期刊介绍：
Drug Metabolism and Personalized Therapy (DMPT) is a peer-reviewed journal, and is abstracted/indexed in relevant major Abstracting Services. It provides up-to-date research articles, reviews and opinion papers in the wide field of drug metabolism research, covering established, new and potential drugs, environmentally toxic chemicals, the mechanisms by which drugs may interact with each other and with biological systems, and the pharmacological and toxicological consequences of these interactions and drug metabolism and excretion. Topics: drug metabolizing enzymes, pharmacogenetics and pharmacogenomics, biochemical pharmacology, molecular pathology, clinical pharmacology, pharmacokinetics and drug-drug interactions, immunopharmacology, neuropsychopharmacology.
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