[新出现的人畜共患肠病原体阿尔伯氏杆菌的分子流行病学]。

Atsushi Hinenoya
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引用次数: 0

摘要

阿尔伯蒂埃希菌是一种新兴的人畜共患肠道病原体,与大肠杆菌密切相关。最近在日本发生了几起由阿尔伯蒂埃希菌引起的食源性暴发,患者人数超过100人。这种细菌携带eae基因,类似于致病性大肠杆菌。其中一些含有志贺毒素2 (stx2a, stx2f)基因,这是肠出血性大肠杆菌(EHEC)的主要毒力因子,表明Stx2产生者可能引起人类严重疾病,如溶血性尿毒综合征。然而,由于缺乏对其细菌学特征和诊断方法的了解,阿尔伯氏杆菌相关感染可能被低估,感染源和途径尚未了解。我们连续进行了针对细胞致死膨胀产毒大肠杆菌的分子流行病学研究,意外地发现cdt-II基因阳性的分离株不是大肠杆菌,而是阿尔伯蒂大肠杆菌。这一发现促使我们开始更多地关注阿尔伯蒂e。我们建立了一种针对Eacdt基因的艾伯氏杆菌特异性PCR检测方法和艾伯氏杆菌选择性分离培养基xrmmacconkey琼脂,为该细菌的检测、分离和鉴定建立了简单、高效、可靠的方法。利用这些方法对野生动物进行调查,发现浣熊是阿尔伯蒂埃氏杆菌的潜在天然宿主。在这里,我描述了我和我的同事们所研究的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Molecular epidemiology of Escherichia albertii, emerging zoonotic enteropathogen].

Escherichia albertii is an emerging zoonotic enteric pathogen, closely related to E. coli. Several foodborne outbreaks caused by E. albertii accounting for >100 patients have recently occurred in Japan. This bacterium carries eae gene, similar to enteropathogenic E. coli. Some of them harbor Shiga toxin 2 (stx2a, stx2f) genes, primary virulence factor of enterohemorrhagic E. coli (EHEC), suggesting that the Stx2 producers could cause severe diseases such as HUS in humans. However, due to lack of the knowledges about its bacteriological characteristics and of the diagnostic methods, E. albertii-related infections might have been underestimated, and the infection sources and routes have not yet been understood. We had continuously performed molecular epidemiological studies targeting for cytolethal distending toxin-producing E. coli, and unexpectedly found that cdt-II gene-positive isolates were not E. coli but E. albertii. This finding led us to initiate research more focusing on E. albertii. We have constructed simple, efficient and reliable methods for the detection, isolation and identification of this bacterium by developing an E. albertii-specific PCR assay targeting Eacdt genes and E. albertii-selective isolation medium named XRM-MacConkey agar. We have also identified raccoons as a potential natural reservoir of E. albertii through wildlife survey using these methods. Here, I describe what I have studied with my colleagues.

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